Natronorubrum halalkaliphilum sp. nov., a haloalkaliphilic archaeon isolated from soda lake in Inner Mongolia Autonomous Region, China

Abstract

A haloalkaliphilic strain JWXQ-INN-674T was isolated from the water sample of a soda lake in Inner Mongolia Autonomous Region, China. Cells of the strain were coccoid, motile, and strictly aerobic. The strain was able to grow in presence of 2.6–5.4 M NaCl (optimum concentration is 3.4 M) at 30–50 °C (optimum temperature is 42 °C) and pH 7–9.5 (optimum pH is 9.0). The 16S rRNA gene sequence of strain JWXQ-INN-674T showed 95.3–96.6% similarity to members of the genus Natronorubrum of the family Natrialbaceae. The whole genome sequencing of strain JWXQ-INN-674T revealed a genome size of 4.56 M bp and a DNA G + C content of 62.5 mol%. Genome relatedness of strain JWXQ-INN-674T and other species in the genus Natronorubrum was analyzed by average nucleotide identity and digital DNA–DNA hybridization with the values of 76.8–90.6 and 23.1–39.3%, respectively. The strain possessed the polar lipids phosphatidylglycerol and methylated phosphatidylglycerol phosphate lipid. No glycolipids were detected. Based on phylogenetic analysis, phenotypic characteristics, chemotaxonomic properties and genome relatedness, the isolate was proposed as the type strain of a novel species of genus Natronorubrum, Natronorubrum halalkaliphilum sp. nov. (type strain JWXQ-INN-674T = CGMCC 1.17283T = JCM 34245T).

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Acknowledgements

We thank “China General Microbiological Culture Collection Center” for deposition of the novel species.

Funding

This study was supported by the National Natural Science Foundation of China (No. 91751201) and the Science and Technology Basic Resources Investigation Project (No. 2017FY100300).

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Correspondence to Dahe Zhao or Hua Xiang.

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The GenBank accession numbers for 16S rRNA gene, rpoB’ gene and genome sequences of strain JWXQ-INN-674T are MN658427, MT814703 and WUYX00000000.

Communicated by Erko Stackebrandt.

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Xue, Q., Zhao, D., Zuo, Z. et al. Natronorubrum halalkaliphilum sp. nov., a haloalkaliphilic archaeon isolated from soda lake in Inner Mongolia Autonomous Region, China. Arch Microbiol (2021). https://doi.org/10.1007/s00203-021-02207-8

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Keywords

  • Natronorubrum
  • Soda lake
  • Haloalkaliphilic archaeon
  • Whole genome sequence