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Schlegelella koreensis sp. nov., isolated from evaporator core of automobile air conditioning system

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Abstract

A white-coloured, aerobic, and rod-shaped bacterium, designated strain ID0723T was isolated from evaporator core of automobile air conditioning system. The strain was Gram-stain-negative, catalase positive, oxidase negative, and grew at pH 5.5–9.5, at temperature 18–37 °C, and at 0–2.0% (w/v) NaCl concentration. The phylogenetic analysis and 16S rRNA gene sequence data revealed that the strain ID0723T was affiliated to the genus Schlegelella, with the closest phylogenetic member being Schlegelella brevitalea DSM 7029 T (98.1% sequence similarity). The chemotaxonomic features of strain ID0723T were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as the main polar lipids; Q-8 as an only ubiquinone; and summed feature 3 (C16:1ω7c and/or C16: 1ω6c), C16:0, and summed feature 8 (C18:1ω7c/or C18:1ω6c) as the major fatty acids. The average nucleotide identity (ANI) and in silico DNA–DNA hybridization values between strain ID0723T and S. brevitalea DSM 7029 T were 74.8% and 20.0%, respectively, which were below the cut-off values of 95% and 70%, respectively. The DNA G + C content was 69.9 mol%. The polyphasic taxonomic data clearly indicated that strain ID0723T represents a novel species in the genus Schlegelella for which the name Schlegelella koreensis sp. nov. is proposed, with the type strain ID0723T (= KCTC 72731 T = NBRC 114611 T).

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Abbreviations

NBRC:

NITE Biological Resource Center

KCTC:

Korean Collection for Type Cultures

ANI:

Average nucleotide identity

References

  • Aziz RK, Bartels D, Best AA et al (2008) The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9:75

    Article  Google Scholar 

  • Blin K, Shaw S, Steinke K et al (2019) antiSMASH 5.0: updates to the secondary metabolite genome mining pipeline. Nucleic Acids Res 47:W81–W87

    Article  CAS  Google Scholar 

  • Breznak JA, Costilow RN (2007) Physicochemical factors in growth. In: Reddy CA, Beveridge TJ, Breznak JA et al (eds) Methods for general and molecular bacteriology, 3rd edn. American Society of Microbiology, Washinton, DC, pp 309–329

    Google Scholar 

  • Chaudhary DK, Kim J (2018) Flavobacterium naphthae sp. nov., isolated from oil-contaminated soil. Int J Syst Evol Microbiol 68:305–309

    Article  CAS  Google Scholar 

  • Chou YJ, Sheu SY, Sheu DS et al (2006) Schlegelella aquatica sp. nov., a novel thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 56:2793–2797

    Article  CAS  Google Scholar 

  • Collins MD, Jones D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45:316–354

    Article  CAS  Google Scholar 

  • Doetsch RN (1981) Determinative methods of light microscopy. In: Gerhardt P, Murray RGE, Costilow RN et al (eds) Manual of methods for general bacteriology. American Society for Microbiology, Washington DC, pp 21–33

    Google Scholar 

  • Elbanna K, Lütke-Eversloh T, Van Trappen S et al (2003) Schlegelella thermodepolymerans gen. nov., sp. nov., a novel thermophilic bacterium that degrades poly(3-hydroxybutyrate-co-3-mercaptopropionate). Int J Syst Evol Microbiol 53:1165–1168

    Article  CAS  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution (N Y) 39:783–791

    Google Scholar 

  • Frank JA, Reich CI, Sharma S et al (2008) Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 74:2461–2470

    Article  CAS  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  Google Scholar 

  • Komagata K, Suzuki KI (1988) 4 Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207

    Article  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  • Lee I, Chalita M, Ha S-M et al (2017) ContEst16S: an algorithm that identifies contaminated prokaryotic genomes using 16S RNA gene sequences. Int J Syst Evol Microbiol 67:2053–2057

    Article  CAS  Google Scholar 

  • Lütke-Eversloh T, Elbanna K, Cnockaert MC et al (2004) Caenibacterium thermophilum is later synonym of Schlegelella thermodepolymerans. Int J Syst Evol Microbiol 54:1933–1935

    Article  Google Scholar 

  • Manaia CM, Nunes OC, Nogales B (2003) Caenibacterium thermophilum gen. nov., sp. nov., isolated from a thermophilic aerobic digester of municipal sludge. Int J Syst Evol Microbiol 53:1375–1382

    Article  CAS  Google Scholar 

  • Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14:60

    Article  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M et al (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Na SI, Kim YO, Yoon SH et al (2018) UBCG: Up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol 56:281–285

    Article  Google Scholar 

  • Pruesse E, Peplies J, Glöckner FO (2012) SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28:1823–1829

    Article  CAS  Google Scholar 

  • Richter M, Rosselló-Móra R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci 106:19126–19131

    Article  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  • Sasser M (1990) Bacterial identification by gas chromatographic analysis of fatty acid methyl esters (GC-FAME). MIDI Tech Note 101 Newwark, MIDI Inc

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654

    Google Scholar 

  • Tang B, Yu Y, Liang J et al (2019) Reclassification of 'Polyangium brachysporum’ DSM 7029 as schlegelella brevitalea sp. Nov Int J Syst Evol Microbiol 69:2877–2883

    Article  CAS  Google Scholar 

  • Tatusova T, DiCuccio M, Badretdin A et al (2016) NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 44:6614–6624

    Article  CAS  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR et al (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 37:463–464

    Article  Google Scholar 

  • Yoon S-H, Ha S, Lim J et al (2017a) A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek 110:1281–1286

    Article  CAS  Google Scholar 

  • Yoon SH, Ha SM, Kwon S et al (2017b) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617

    Article  CAS  Google Scholar 

  • Zhang Z, Schwartz S, Wagner L, Miller W (2000) A greedy algorithm for aligning DNA sequences. J Comput Biol 7:203–214

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by Rural Development Administration (Project No. PJ014897032020) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019R1A6A3A01091574).

Funding

The GenBank/EMBL/DDBJ Accession number for the 16S rRNA sequence of strain ID0723T is KP326334. The whole genome shotgun sequence of strain ID0723T has been deposited at DDBJ/ENA/GenBank under the Accession JABWMJ000000000. The version described in this paper is version JABWMJ010000000.

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Author notes

  1. Dhiraj Kumar Chaudhary and Hyosun Lee contributed equally to this study.

Authors and Affiliations

  1. Department of Environmental Engineering, College of Science and Technology, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, 30019, Republic of Korea

    Dhiraj Kumar Chaudhary

  2. Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, 26339, Republic of Korea

    Hyosun Lee & Dong-Uk Kim

  3. Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, 41944, Republic of Korea

    Ram Hari Dahal

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  1. Dhiraj Kumar Chaudhary
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  2. Hyosun Lee
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  4. Dong-Uk Kim
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Correspondence to Dong-Uk Kim.

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This study does not describe any experimental work related to human.

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Communicated by Erko Stackebrandt.

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Chaudhary, D.K., Lee, H., Dahal, R.H. et al. Schlegelella koreensis sp. nov., isolated from evaporator core of automobile air conditioning system. Arch Microbiol 203, 2373–2378 (2021). https://doi.org/10.1007/s00203-021-02206-9

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  • DOI: https://doi.org/10.1007/s00203-021-02206-9

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