Skip to main content
SpringerLink
Log in

Adhesion and colonization of potential probiont Pseudomonas aeruginosa FARP72 in the intestine of yellowtail catfish, Pangasius pangasius

  • Short Communication
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Adhesion is recognized as the first important step of a probiont for intestinal colonization. This study assessed the ability of an antagonistic Pseudomonas aeruginosa FARP72 to adhere and colonize the intestine of yellowtail catfish, Pangasius pangasius both in vitro and in vivo. For the in vitro assay, the whole intestines of each of two starved P. pangasius were introduced separately into tubes containing bluish-green pigment-producing P. aeruginosa FARP72 at 8.00 log10 CFU/mL and physiological saline (0.85% sodium chloride) and incubated for 1 h at 30 ± 1 °C. The homogenate mucus solutions from the intestine samples were serially diluted and plated onto Pseudomonas isolation agar to determine the counts of bluish-green pigment-producing P. aeruginosa (BPPAC). The difference between the numbers of BPPAC and presumptive Pseudomonas counts (PPC) in the treated and control intestines was attributed to the adherence of P. aeruginosa FARP72. The levels of BPPAC and PPC in the treated intestines were 6.09 ± 0.59 log10 CFU/g. Similarly, following 30 days of feeding P. pangasius with P. aeruginosa FARP72 supplemented diet, the intestine of catfish recorded the BPPAC of 5.83 ± 0.64 log CFU/g. In control samples, the BPPACs were recorded as < 3.00 log10 CFU/g. The scanning electron micrograph of the intestines of P. pangasius following the in vitro and in vivo adhesion assays confirmed the ability of this bacterium to strongly adhere to the intestine, thus making it most suitable candidate probiont for use in freshwater catfish aquaculture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Availability of data and materials

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Code availability

Not applicable.

References

  • Balcazar JL, Vendrell D, Blas ID, Ruiz-Zarzuela I, Girones O, Muzquiz JL (2008) In-vitro competitive adhesion and production of antagonistic compounds by lactic acid bacteria against fish pathogens. Vet Microbiol 122(3–4):373–380

    Google Scholar 

  • Collado MC, Meriluoto J, Salminen S (2008) Adhesion and aggregation properties of probiotic and pathogen strains. Eur Food Res Technol 226:1065–1073

    Article  CAS  Google Scholar 

  • Ekmekci H, Aslim B, Ozturk S (2009) Characterization of vaginal lactobacilli coaggregation ability with Escherichia coli. Microbiol Immunol 53(2):59–65

    Article  CAS  Google Scholar 

  • Ferguson RM, Merrifield DL, Harper GM, Rawling MD, Mustafa S, Picchietti S, Balcázar JL, Davies SJ (2010) The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus). J Appl Microbiol 109(3):851–862

    Article  CAS  Google Scholar 

  • Fernandes IM, Bastos YF, Barreto DS, Lourenço LS, Penha JM (2017) The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes. Braz J Biol 77(3):444–450

    Article  CAS  Google Scholar 

  • Ghosh K, Roy M, Kar N, Ringo E (2010) Gastrointestinal bacteria in rohu, Labeo rohita scanning electron microscopy and bacteriological study. Acta Ichthyol Piscat 40(2):129–135

    Article  Google Scholar 

  • Hoque F (2015) Screening and characterisation of antagonistic Pseudomonas aeruginosa FARP72 as a potential probiotic agent. Indian J Fish 62(4):80–90

    Google Scholar 

  • Hoque F, Abraham TJ, Nagesh TS, Kamilya D (2019) Pseudomonas aeruginosa FARP72 offers protection against Aeromonas hydrophila infection in Labeo rohita. Probiotics Antimicrob Proteins 11(3):973–980

    Article  Google Scholar 

  • Ibrahim F, Ouwehand AC, Salminen SJ (2004) Effect of temperature on in vitro adhesion of potential fish probiotics. Microb Ecol Health Dis 16(4):222–227

    CAS  Google Scholar 

  • Kesarcodi-Watson A, Kaspar H, Lategan MJ, Gibson L (2008) Probiotics in aquaculture: the need principles and mechanisms of action and screening processes. Aquaculture 274:1–14

    Article  Google Scholar 

  • Kos B, Suskovic J, Vukovic S, Simpraga M, Frece J, Matosic S (2003) Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J Appl Microbiol 94:981–987

    Article  CAS  Google Scholar 

  • Lehata S, Inagaki T, Okunishi S, Nakano M, Tanaka R, Maeda H (2010) Improved gut environment of abalone Haliotis gigantea through Pediococcus sp. Ab1 treatment. Aquaculture 305:59–65

    Article  Google Scholar 

  • Magariños B, Romalde JL, Noya M, Barja JL, Toranzo AE (1996) Adherence and invasive capacities of the fish pathogen Pasteurella piscicida. FEMS Microbiol Lett 138(1):29–34

    Article  Google Scholar 

  • Merrifield W, Dimitroglou A, Foey A, Davies SJ, Baker RTM, Bøgwald J, Castex M, Ringø E (2010) The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture 302(1–2):1–18

    Article  Google Scholar 

  • Monteagudo-Mera A, Rastall RA, Gibson GR, Charalampopoulos D, Chatzifragkou A (2019) Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health. Appl Microbiol Biotechnol 103:6463–6472

    Article  CAS  Google Scholar 

  • Nikoskelainen S, Salminen S, Bylund G, Ouwehand A (2001) Characterization of the properties of human and dairy-derived probiotics for prevention of infectious diseases in fish. Appl Environ Microbiol 67:2430–2435

    Article  CAS  Google Scholar 

  • Ouwehand AC, Kirjavainen PV, Gronlund MM, Isolauri E, Salminen S (1999) Adhesion of probiotic microbiotic microorganisms to intestinal mucus. Int Dairy J 9(9):623–630

    Article  Google Scholar 

  • Pan X, Wu T, Zhang L, Song Z, Tang H, Zhao Z (2008) In vitro evaluation on adherence and antimicrobial properties of a candidate probiotic Clostridium butyricum CB2 for farmed fish. J Appl Microbiol 105:1623–1629

    Article  CAS  Google Scholar 

  • Planas M, Perez-Lorenzo M, Hjelm M, Gram L, Fiksdal IU, Bergh Ø (2006) Probiotic effect in vivo of Roseobacter strain 27–4 against Vibrio (Listonella) anguillarum infections in turbot (Scophthalmus maximus L.) larvae. Aquaculture 255(1–4):323–333

    Article  Google Scholar 

  • Ringø E, Olsen RE (1999) The effect of diet on aerobic bacterial flora associated with intestine of Arctic charr (Salvelinus alpinus L.). J Appl Microbiol 86(1):22–28

    Article  Google Scholar 

  • Ringø E, Olsen RE, Mayhew TM, Myklebust R (2003) Electron microscopy of the intestinal microflora of fish. Aquaculture 227:395–415

    Article  Google Scholar 

  • Ringø E, Wesmajervi MS, Bendiksen HR, Berg A, Olsen RE, Johnsen T et al (2001) Identification and characterization of carnobacteria isolated from fish intestine. Syst Appl Microbiol 24:183–191

    Article  Google Scholar 

  • Rinkinen M, Matto J, Salminen S, Westermarck E, Ouwehand AC (2000) In vitro adhesion of lactic acid bacteria to canine small intestinal mucus. J Anim Physiol Anim Nutr 84:43–47

    Article  Google Scholar 

  • Schaber JA, Hammond A, Carty NL, Williams SC, Colmer-Hamood JA, Burrowes BH, Dhevan V, Griswold JA, Hamood AN (2007) Diversity of biofilms produced by quorum-sensing deficient clinical isolates of Pseudomonas aeruginosa. J Med Microbiol 56:738–748

    Article  CAS  Google Scholar 

  • Sugimura Y, Hagi T, Hoshino T (2011) Correlation between in vitro mucus adhesion and the in vivo colonization ability of lactic acid bacteria: screening of new candidate carp probiotics. Biosci Biotechnol Biochem 75(3):511–515

    Article  CAS  Google Scholar 

  • Thomas-Jinu S, Goodwin AE (2004) Acute columnaris infection in channel catfish Ictalurus punctatus (Rafinesque): efficacy of practical treatments for warmwater aquaculture ponds. J Fish Dis 27(1):23–28

    Article  CAS  Google Scholar 

  • Tuomola EM, Salminen SJ (1998) Adhesion of some probiotic and dairy Lactobacillus strains to Caco-2 cell cultures. Int J Food Microbiol 41(1):45–51

    Article  CAS  Google Scholar 

  • Verschuere L, Rombaut G, Sorgeloos P, Verstraete W (2000) Probiotic bacteria as biolog10ical control agents in aquaculture. Microbiol Mol Biol Res 64(4):655–671

    Article  CAS  Google Scholar 

  • Villamil L, Tafalla C, Figueras A, Novoa B (2002) Evaluation of immunomodulatory effects of lactic acid bacteria in turbot (Scophthalmus maximus). J Clin Diag Lab Immunol 9(6):1318–1323

    CAS  Google Scholar 

  • Vine NG, Leukes WD, Kaiser H (2004) In vitro growth characteristics of five candidate aquaculture probiotics two fish pathogens grown in fish intestinal mucus. FEMS Lett Microbiol 231(1):145–152

    Article  CAS  Google Scholar 

  • Wang Y, Ren Z, Fu L, Su X (2016) Two highly adhesive lactic acid bacteria strains are protective in zebrafish infected with Aeromonas hydrophila by evocation of gut mucosal immunity. J Appl Microbiol 120(2):441–451

    Article  CAS  Google Scholar 

  • Yousefian M, Amiri MS (2009) A review of the use of prebiotic in aquaculture for fish and shrimp. African J Biotechnol 8(25):7313–7318

    CAS  Google Scholar 

Download references

Funding

This research was supported by the Department of Science and Technology (DST), Ministry of Science and Technology, New Delhi, Govt. of India under the scheme "Innovation in Science Pursuit for Inspired Research (INSPIRE)", Fellowship No. IF140455.

Author information

Author notes

  1. Farhana Hoque

    Present address: Regional Research Station, ICAR-Central Institute of Freshwater Aquaculture, Rahara, Kolkata, 700118, West Bengal, India

Authors and Affiliations

  1. Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata, 700094, West Bengal, India

    Farhana Hoque & T. Jawahar Abraham

Authors
  1. Farhana Hoque
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Jawahar Abraham
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Farhana Hoque.

Ethics declarations

Ethics approval

Authors of this paper have read and approved the final version submitted. The contents of this manuscript have not been copyrighted or published previously. (1) The contents of this manuscript are not now under consideration for publication elsewhere. (2) The contents of this manuscript will not be copyrighted, submitted, or published elsewhere, while acceptance by the Journal is under consideration. (3) Use of laboratory animals (fish) in the present study has complied with the guidelines and policies of the ethical committee of the Institute.

Consent to participate

Not applicable.

Consent for publication

All authors have given consent for publication of this manuscript.

Conflict of interest

The authors declare that there is no conflict of interest.

Additional information

Communicated by Erko Stackebrandt.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hoque, F., Abraham, T.J. Adhesion and colonization of potential probiont Pseudomonas aeruginosa FARP72 in the intestine of yellowtail catfish, Pangasius pangasius. Arch Microbiol 203, 2711–2717 (2021). https://doi.org/10.1007/s00203-021-02188-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-021-02188-8

Keywords

Search

Navigation