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Haloechinothrix aidingensis sp. nov., an actinomycete isolated from salt lake in Xinjiang province, north-west China

Archives of Microbiology volume 203, pages 1801–1806 (2021)Cite this article

Abstract

An actinomycete strain, designated YIM 98757T, was isolated from the hypersaline sediment of Aiding Lake in Xinjiang province, north-west China. The strain grew well on most media tested and no diffusible pigment was produced. The substrate mycelium was well developed and fragmented. No spores were formed. The whole-cell hydrolysates contained meso-diaminopimelic acid as the cell-wall diamino acid. Xylose, galactose, ribose were the major whole-cell sugars. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unknown phospholipid. The predominant menaquinone was MK-8(H4). The major fatty acid was iso-C16:0. The DNA G + C content was 69.1 mol%. Phylogenetic analysis indicated that the isolate belongs to the genus Haloechinothrix. However, it differed from its closest relative, H. alba YIM 98757 T in many phenotypic and chemotaxonomic characteristics. Moreover, the DNA–DNA and ANI relatedness values between the novel isolate and H. alba YIM 93221 T were 53.3% and 92.5%, respectively. Based on comparative analysis of polyphasic taxonomic data, strain YIM 98757 T represents a novel species of the genus Haloechinothrix, for which the name Haloechinothrix aidingensis sp. nov. is proposed. The type strain is YIM 98757T (= CGMCC 4.7627T = CCTCC AA 2020012).

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Fig. 1

Abbreviations

CA:

Czapek’s agar

ISP 5:

Glycerol/asparagine agar

ISP 4:

Inorganic salts-starch agar

NA:

Nutrient agar

OA:

Oatmeal agar

PA:

Potato agar

ISP2:

Yeast extract-malt extract agar

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

PE:

Phosphatidylethanolamine

PL:

Unknown phospholipid

PI:

Phosphotidylinositol

PIM:

Phosphotidylinositol dimannoside

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Acknowledgements

This study was performed with support of the National Natural Science Foundation of China (31760003, 31500011, 31800001 and 31860017), the Natural Science Foundation of Guangdong Province (2017A030310206), the South and Southeast Asia Cooperation Base on Microbiological Resource Prevention and Utilization (2018IA100) and Chunhui Project for Ministry of Education of China (Project No. 191649) and Major Science and Technology Projects of Yunnan Province (Digitalization, development and application of biotic resource, 202002AA100007).

Funding

The NCBI GenBank accession number for the 16S rRNA gene sequence of strain YIM 98757T is MN251022. The draft whole genome sequence for YIM 98757T has been deposited at DDBJ/ENA/GenBank under accession number GCA_013450155.1.

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Author notes

  1. Li Yang and Ya-Rong Kou equally contributed to this work.

Affiliations

  1. Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, and Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, 650091, P. R. China

    Li Yang, Ya-Rong Kou, Yong-Jiang Shi, Wen-Yong Zhu & Shu-Kun Tang

  2. Yunnan Key Laboratory of Vaccine Research & Development On Severe Infections Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, P. R. China

    Wen-Yong Zhu

  3. School of Medicine, Yunnan University, Kunming, 650091, P. R. China

    Min Yin

  4. College of Food and Biological Engineering, Xihua University, Chengdu, 610039, P. R. China

    Tong-Wei Guan

  5. Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, College of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang, 473004, P. R. China

    Bing-Bing Liu

  6. Walvax Biotechnology Co., Ltd, Kunming, 650106, P. R. China

    Ya-Rong Kou

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  1. Li Yang
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Contributions

LY and YRK carried out the data analysis, part of chemical classification, and wrote the manuscript. TWG and MY performed the polyphasic taxonomy except chemical classification. BBL provided the soil samples. YJS prepared the experiments and isolated the novel strain. WYZ and SKT designed the separation medium and directed the classification and takes full responsibility for the final submission. All authors reviewed and approved the final version of the paper.

Corresponding authors

Correspondence to Wen-Yong Zhu or Shu-Kun Tang.

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Yang, L., Kou, YR., Guan, TW. et al. Haloechinothrix aidingensis sp. nov., an actinomycete isolated from salt lake in Xinjiang province, north-west China. Arch Microbiol 203, 1801–1806 (2021). https://doi.org/10.1007/s00203-021-02184-y

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Keywords

  • Haloechinothrix aidingensis
  • sp. nov.
  • Salt lake
  • Polyphasic taxonomy