Reclassification of Sphaerotilus natans subsp. sulfidivorans Gridneva et al. 2011 as Sphaerotilus sulfidivorans sp. nov. and comparative genome analysis of the genus Sphaerotilus

Grabovich, Margarita Y.; Smolyakov, Dmitry D.; Beletsky, Alexey V.; Mardanov, Andrey V.; Gureeva, Maria V.; Markov, Nikita D.; Rudenko, Tatyana S.; Ravin, Nikolai V.

doi:10.1007/s00203-020-02158-6

Original Paper
Published: 05 January 2021

Reclassification of Sphaerotilus natans subsp. sulfidivorans Gridneva et al. 2011 as Sphaerotilus sulfidivorans sp. nov. and comparative genome analysis of the genus Sphaerotilus

Archives of Microbiology volume 203, pages 1595–1599 (2021)Cite this article

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Abstract

Filamentous iron oxides accumulating bacteria Sphaerotilus natans subsp. natans and S. natans subsp. sulfidivorans were described as subspecies based on 99.7% identity of their 16S rRNA sequences, in spite of important physiological difference. The ANI between their genomes was 94.7%, which indicate their assignment to different species. S. natans subsp. sulfidivorans and S. montanus possess genes for a complete SOX system, while S. natans subsp. natans encode only SoxYZ. There are genes for the Calvin cycle in the genomes of S. hippei DSM 566^T, S. natans subsp. sulfidivorans D-501^T, and S. montanus HS^T. Lithoautotrophy on reduced sulfur compounds is probably possible for S. natans subsp. sulfidivorans and S. montanus, but not for S. natans subsp. natans. Considering significant differences in the genome characteristics and metabolic potential of S. natans subsp. sulfidivorans and S. natans subsp. natans, we propose their classification as different species, S. natans and S. sulfidivorans sp. nov.

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Funding

This work was partly supported by the Russian Science Foundation [project 20-14-00137 to MYG].

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Affiliations

Department of Biochemistry and Cell Physiology, Voronezh State University, 1 Universitetskaya pl, Voronezh, 394018, Russia
Margarita Y. Grabovich, Dmitry D. Smolyakov, Maria V. Gureeva, Nikita D. Markov & Tatyana S. Rudenko
Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia
Alexey V. Beletsky, Andrey V. Mardanov & Nikolai V. Ravin

Authors

Margarita Y. Grabovich
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Dmitry D. Smolyakov
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Alexey V. Beletsky
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Andrey V. Mardanov
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Maria V. Gureeva
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Nikita D. Markov
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Tatyana S. Rudenko
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Nikolai V. Ravin
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Contributions

Conceptualization, validation, and project administration: MYG and NVR; methodology, investigation, and writing—original draft preparation: DDS; methodology, investigation: NDM, AVB, and AVM; writing—review and editing: MVG, TSR, and NVR.

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Correspondence to Margarita Y. Grabovich.

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Communicated by Erko Stackebrandt.

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Grabovich, M.Y., Smolyakov, D.D., Beletsky, A.V. et al. Reclassification of Sphaerotilus natans subsp. sulfidivorans Gridneva et al. 2011 as Sphaerotilus sulfidivorans sp. nov. and comparative genome analysis of the genus Sphaerotilus. Arch Microbiol 203, 1595–1599 (2021). https://doi.org/10.1007/s00203-020-02158-6

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Received: 26 October 2020
Revised: 01 December 2020
Accepted: 14 December 2020
Published: 05 January 2021
Issue Date: May 2021
DOI: https://doi.org/10.1007/s00203-020-02158-6

Keywords

Sphaerotilus
Sphaerotilus natans subsp. sulfidivorans
Sphaerotilus sulfidivorans sp. nov.
ANI
AAI
Digital DDH