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Expression and in vitro anticancer activity of Lp16-PSP, a member of the YjgF/YER057c/UK114 protein family from the mushroom Lentinula edodes C91-3

Archives of Microbiology volume 203, pages 1047–1060 (2021)Cite this article

Abstract

Latcripin-16 (Lp16-PSP) is a gene that was extracted as a result of de novo characterization of the Lentinula edodes strain C91-3 transcriptome. The aim of the present study was to clone, express, and investigate the selective in vitro anticancer potential of Lp16-PSP in human cell lines. Lp16-PSP was analyzed using bioinformatics tools, cloned in a prokaryotic expression vector pET32a (+) and transformed into E. coli Rosetta gami. It was expressed and solubilized under optimized conditions. The differential scanning fluorometry (DSF)-guided refolding method was used with modifications to identify the proper refolding conditions for the Lp16-PSP protein. To determine the selective anticancer potential of Lp16-PSP, a panel of human cancerous and non-cancerous cell lines was used. Lp16-PSP protein was identified as endoribonuclease L-PSP protein and a member of the highly conserved YjgF/YER057c/UK114 protein superfamily. Lp16-PSP was expressed under optimized conditions (37 °C for 4 h following induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside). Solubilization was achieved with mild solubilization buffer containing 2 M urea using the freeze–thaw method. The DSF guided refolding method identified the proper refolding conditions (50 mM Tris–HCl, 100 mM NaCl, 1 mM EDTA, 400 mM Arginine, 0.2 mM GSH and 2 mM GSSG; pH 8.0) for Lp16-PSP, with a melting transition of ~ 58 °C. A final yield of ~ 16 mg of purified Lp16-PSP from 1 L of culture was obtained following dialysis and concentration by PEG 20,000. A Cell Counting Kit-8 assay revealed the selective cytotoxic effect of Lp16-PSP. The HL-60 cell line was demonstrated to be most sensitive to Lp16-PSP, with an IC50 value of 74.4 ± 1.07 µg/ml. The results of the present study suggest that Lp16-PSP may serve as a potential anticancer agent; however, further investigation is required to characterize this anticancer effect and to elucidate the molecular mechanism underlying the action of Lp16-PSP.

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Acknowledgements

The authors would like to thank Richardson Patrick Joseph for editorial assistance for his generous support, insightful comments, and suggestions.

Funding

The present study was supported by a Chinese Government Scholarship (CSC No. 2014GXY960), National Natural Science Foundation of China (Grant No. 81472836) and the Liaoning Provincial Program for Top Discipline of College of Basic Medical Sciences.

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  1. Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, 9 West Section, Lvshun South Road, Luvshoukon District, Dalian, 116044, Liaoning, People’s Republic of China

    Thomson Patrick Joseph, Warren Chanda, Yukun Fang, MinTao Zhong & Min Huang

  2. Center for Neuroscience, Shantou University Medical College, Shantou, People’s Republic of China

    Thomson Patrick Joseph

  3. Computational System Biology Laboratory, Department of Bioinformatics, Shantou University Medical College, Shantou, People’s Republic of China

    Qianqian Zhao

  4. Department of Biotechnology, College of Basic Medical Sciences, Dalian Medical University, Dalian, 116044, Liaoning, People’s Republic of China

    Sadia Kanwal

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TPJ designed the study and performed experiments. QZ, WC and SK designed the study and participated in the analysis of results, review and editing of the manuscript. FYK participated in the analysis of results, review and editing of the manuscript. MTZ has contributed in the project administration, interpretation of results and revision of the manuscript. The final manuscript was approved by MH.

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Joseph, T.P., Zhao, Q., Chanda, W. et al. Expression and in vitro anticancer activity of Lp16-PSP, a member of the YjgF/YER057c/UK114 protein family from the mushroom Lentinula edodes C91-3. Arch Microbiol 203, 1047–1060 (2021). https://doi.org/10.1007/s00203-020-02099-0

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Keywords

  • Lentinula edodes C91-3
  • Latcripin-16
  • Endoribonuclease
  • HL-60 cell line