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Production of antibiotic carbomycin from Streptomyces graminofaciens with high lipid content mutation

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Abstract

The most important tools in killing and overcoming on the microbes and pathogens that cause diseases in medicine and/or in agriculture are the antibiotics. The discovery and synthesis of the microbial natural products or antibiotics has greatly developed genetically and biotechnologically quickly in the last decades. It is necessary to access this great genetic diversity by finding ways to increase the level of expression of these biosynthetic pathways. In this study, we carried out an improvement in the antibiotic production of weak Streptomyces graminofaciens strain NBR9 that has high lipid content; using Ultra-Violet irradiation mutagenesis. This strain was isolated from the Northern Region in the kingdom of Saudi Arabia and identified biochemically and confirmed genetically by sequencing of the 16S rRNA gene as Streptomyces graminofaciens NBR9; Accession No. (MN640578). The resultant mutant strain showed increasing in their antimicrobial activities. The methods and techniques used for the antibiotic extraction, purification, characterization and identification proved that the obtained antibiotic is same with antibiotic Carbomycin.

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Acknowledgements

The authors gratefully acknowledge the approval and the support of this research study by the Grant No. (SAR-2018-3-9-F-7709) from the Deanship of Scientific Research at Northern Border University, Arar, KSA.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science and Arts, Northern Border University, Rafha, Saudi Arabia

    Hussam Hassan Arafat & Medhat Ahmed Abu-Tahon

  2. Department of Botany and Microbiology, Faculty of Science, Minia University, Minia City, 61519, Egypt

    Hussam Hassan Arafat

  3. Biological and Geological Sciences Department, Faculty of Education, Ain Shams University, Roxy, Heliopolis, P.C.11757, Cairo, Egypt

    Medhat Ahmed Abu-Tahon & George Saad Isaac

Authors
  1. Hussam Hassan Arafat
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  2. Medhat Ahmed Abu-Tahon
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  3. George Saad Isaac
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Correspondence to Hussam Hassan Arafat.

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Communicated by Erko Stackebrandt.

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Arafat, H.H., Abu-Tahon, M. & Isaac, G.S. Production of antibiotic carbomycin from Streptomyces graminofaciens with high lipid content mutation. Arch Microbiol 203, 901–911 (2021). https://doi.org/10.1007/s00203-020-02085-6

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  • DOI: https://doi.org/10.1007/s00203-020-02085-6

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