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Chryseobacterium cheonjiense sp. nov., isolated from forest soil

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Abstract

A yellow-pigmented, non-motile and rod-shaped bacterium, designated RJ-7-14T was obtained from forest soil sampled at Cheonji-dong, Seogwipo-si, Jeju-do, South Korea. Cells were Gram-stain-negative and produced flexirubin type pigments. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RJ-7-14T formed a lineage within the family Weeksellaceae and clustered as members of the genus Chryseobacterium. The closest members were Chryseobacterium geocarposphaerae DSM 27617T (98.2% sequence similarity), Chryseobacterium hispalense DSM 25574T (98.0%) and Chryseobacterium nepalense KACC 18907T (98.0%). The sequence similarity for other members was < 98.0%. The genome was 4,276,416 bp long with 9 scaffolds and 3779 protein-coding genes. The sole respiratory quinone was MK-6. The major cellular fatty acids were iso-C15:0, summed feature 9 (iso-C17:1 ω9c and/or C16:0 10-methyl), summed feature 3 (iso-C15:0 2-OH and/or C16: 1ω7c) and iso-C17:0 3-OH. The major polar lipid was phosphatidylethanolamine (PE). The DNA G + C content of the type strain was 37.2 mol%. In addition, the average nucleotide identity (ANIu) and in silico DNA–DNA hybridization (dDDH) relatedness values between strain RJ-7-14T and phylogenetically closest members were ≤ 88.2% and ≤ 35.0%, respectively, which were below the threshold values of 95–96% (for ANI) and 70% (for dDDH), suggesting the allocation of novel strain to a new species. Based on genomic, chemotaxonomic, phenotypic and phylogenetic analyses, strain RJ-7-14T represents novel species in the genus Chryseobacterium, for which the name Chryseobacterium cheonjiense sp. nov. is proposed. The type strain is RJ-7-14T (= KACC 21625T = NBRC 114362T).

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Acknowledgements

We greatly appreciate Professor Aharon Oren (The Hebrew University of Jerusalem, Israel) for his expert suggestions concerning the correct species epithet and etymology. We thank to Mr. Byungjo Lee (Dongguk University, Republic of Korea) for UBGC tree construction.

Funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF-2019R1F1A1058501).

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Authors and Affiliations

  1. Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do, 16227, Republic of Korea

    Dhiraj Kumar Chaudhary, Ram Hari Dahal & Jaisoo Kim

  2. Department of Environmental Engineering, College of Science and Technology, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, 30019, Republic of Korea

    Dhiraj Kumar Chaudhary

  3. Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, Republic of Korea

    Dong-Uk Kim

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GenBank/EMBL/DDBJ accessions of the 16S rRNA gene sequences for strain RJ-7-14T is MN661191 and Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JABBGF000000000. The version described in this manuscript is version JABBGF010000000.

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Chaudhary, D.K., Dahal, R.H., Kim, DU. et al. Chryseobacterium cheonjiense sp. nov., isolated from forest soil. Arch Microbiol 203, 725–731 (2021). https://doi.org/10.1007/s00203-020-02065-w

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