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Lentibacillus saliphilus. sp. nov., a moderately halophilic bacterium isolated from a saltern in Korea

Archives of Microbiology volume 203, pages 621–627 (2021)Cite this article

Abstract

A novel moderately halophilic bacterial strain, designated YIM 93176T, was isolated from a saltern in Korea and subjected to a polyphasic taxonomic study. This isolate YIM 93176T was observed to grow in the presence of 0–22% (w/v) NaCl and at pH 6.0–10.0 and 10–45 °C; optimum growth was observed with 5–10% (w/v) NaCl and at pH 7.0–9.0 and 28–37 °C. Based on 16S rRNA gene sequences analysis, the nearest relatives were Lentibacillus alimentarius M2024T (96.5% similarity), followed by Virgibacillus carmonensis LMG 20964T (96.0%) and the other type strains of the family Bacillaceae, but phylogenetic analysis indicated that strain YIM 93176T belonged to the cluster comprising type species of the genus Lentibacillus. Genome sequencing of strain YIM 93176T revealed a genome size of 3.2 Mb and a DNA G + C content of 40.5 mol%. The major fatty acids were anteiso-C15:0 (40.7%) and iso-C15:0 (26.4%), while the predominant respiratory quinone was menaquinone 7. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. These genotypic and chemotaxonomic characteristics supported affiliation of strain YIM 93176T to the genus Lentibacillus. In addition, phenotypic characteristics could distinguish strain YIM 93176T from its closely related species in genus Lentibacillus. Based on the cumulative evidences from the polyphasic taxonomic study, strain YIM 93176T represents a novel species of the genus Lentibacillus, for which name Lentibacillus saliphilus sp. nov. (type strain YIM 93176T = CCTCC AB 208139T = DSM 21375T) is proposed.

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Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (no. 31400438 and 31760003), Nanhai Marine Biomedical Resources R&D Public Service Platform (no. 2017 C8B2) and the Scientific Research Foundation of Lingnan Normal University (no. 1170919630).

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Affiliations

  1. Life Science and Technology School, Lingnan Normal University, Zhanjiang, 524048, People’s Republic of China

    Yun Wang, Hong-Ping Lin, Peng Sun, Hong-Yan Zhang, Dong-Mei Lu & Li-Yun Wang

  2. Urumqi Customs Technology Center, Urumqi, 830063, People’s Republic of China

    Gang-Qiang Jiang

  3. Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, and Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, 650091, People’s Republic of China

    Shu-Kun Tang

  4. Microbial Resource Center, KRIBB, Daejeon, 305-806, Republic of Korea

    Chang-Jin Kim

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  1. Yun Wang
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Correspondence to Gang-Qiang Jiang or Shu-Kun Tang.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strains YIM 93176T is KY697304. The draft whole genome sequence for YIM 93176T has been deposited at DDBJ/ENA/GenBank under accession number JABJXD000000000.

Communicated by Erko stackebrandt.

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Wang, Y., Jiang, GQ., Lin, HP. et al. Lentibacillus saliphilus. sp. nov., a moderately halophilic bacterium isolated from a saltern in Korea. Arch Microbiol 203, 621–627 (2021). https://doi.org/10.1007/s00203-020-02043-2

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Keywords

  • Lentibacillus saliphilus sp. nov.
  • Saltern sediment
  • Polyphasic taxonomy