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Lysobacter telluris sp. nov., isolated from Korean rhizosphere soil

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Abstract

A Gram-stain-negative, aerobic, non-motile, non-spore-forming light-yellow-coloured rod-shaped bacterial strain, designated YJ15T, was isolated from soil at Bigeum island in Korea. Growth was observed at 10–37 °C (optimum, 28 °C), at pH 6.0–7.5 (optimum, pH 7.0) and in the absence of NaCl. Based on 16S rRNA gene sequence analysis, strain YJ15T was closely related to ‘Lysobacter tongrenensis’ YS037T (97.8%), Lysobacter pocheonensis Gsoil193T (96.5%) and Lysobacter daecheongensis Dae08T (95.8%) and phylogenetically grouped together with ‘Lysobacter tongrenensis’ YS037T, Lysobacter dokdonensis DS-58T and Lysobacter pocheonensis Gsoil 193T. The DNA–DNA relatedness between strain YJ15T and ‘Lysobacter tongrenensis’ KCTC 52206T was 12% and the phylogenomic analysis based on the whole genome sequence demonstrated that strain YJ20T formed a distinct phyletic line with Lysobacterlter dokdonensis DS-58T showing average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of 76.3 and 21.3%, respectively. The predominant ubiquinone was identified as Q-8, and polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified aminolipids. The major fatty acids were iso-C17:1 ω9c, iso-C15:0, iso-C16:0 and iso-C17:0. The genomic DNA G + C content was 68.2 mol %. On the basis of phenotypic, chemotaxonomic properties and phylogenetic analyses in this study, strain YJ15T is considered to represent a novel species of the genus Lysobacter, for which the name Lysobacter telluris sp. nov. is proposed. The type strain is YJ15T (= KACC 19552T = NBRC 113197T).

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Funding

This work was funded by the Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (as part of the Genome Technology to Business Translation Program). grant number 918016-4.

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Authors and Affiliations

  1. Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon, 35349, Republic of Korea

    Jae-Chan Lee & Kyung-Sook Whang

  2. Department of Microbiology & Resources, College of Science & Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon, 35349, Republic of Korea

    Jae-Chan Lee & Kyung-Sook Whang

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  1. Jae-Chan Lee
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Correspondence to Kyung-Sook Whang.

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Communicated by Erko stackebrandt.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YJ15T is JN848797. The whole genome sequence has been deposited at GenBank/ENA/DDBJ under the accession JAANOY000000000. The version described in this paper is version JAANOY000000000.1.

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Lee, JC., Whang, KS. Lysobacter telluris sp. nov., isolated from Korean rhizosphere soil. Arch Microbiol 203, 287–293 (2021). https://doi.org/10.1007/s00203-020-02032-5

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