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Pharmacological properties of some mangrove sediment-associated bacillus isolates

Abstract

Mangrove sediment-associated bacteria are of significantly important in the field of medicine and pharmaceuticals as new promising sources of biologically active pharmacophores due to the extreme conditions, such as high salt concentration and soil anoxia. The sediment bacteria associated with Acanthus ilicifolius and Avicennia officinalis collected from the Mangalavanam mangrove ecosystem of the Kerala State of India were evaluated using various in vitro models for the assessment of their pharmacological properties. The bacteria exhibiting significant antioxidant and antimicrobial activities were isolated, identified, and characterized by the integrated microbiological, biochemical, and 16S rRNA sequencing. Among the varied bacteria isolated from mangrove sediments, Bacillus amyloliquefaciens MBMS5 (GenBank accession number MK765025) exhibited significant antimicrobial activities against various pathogenic bacteria, such as Aeromonas caviae, Vibrio parahemolyticus, and methicillin-resistant Staphylococcus aureus. The extracellular extracts of B. amyloliquefaciens MBMS5 exhibited potential antioxidant activity against free radical species coupled with anti-inflammatory property as displayed by the attenuation activity against pro-inflammatory 5-lipoxygenase.

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Acknowledgements

This work was funded by the College of Agriculture of Kerala Agricultural University, India under the B.Sc.–M.Sc. (Integrated) Biotechnology programme (Grant no. 2014-09-103). The authors are thankful to Indian Council of Agricultural Research (ICAR), New Delhi for providing facilities to carry out the work. The authors thank the Director, Central Marine Fisheries Research Institute, Course Director {B.Sc.–M.Sc. (Integrated) Biotechnology programme} and Head, Department of Plant Biotechnology, College of Agriculture, Kerala Agricultural University, Thiruvananthapuram, for providing with necessary support. Thanks are due to the Head, Marine Biotechnology Division, Central Marine Fisheries Research Institute for facilitating the research activities.

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Correspondence to Kajal Chakraborty.

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This article does not contain any studies with human participants or animals performed by any of the authors. Appropriate permissions were obtained from the responsible authorities for collecting mangrove sediments in the Mangalavanam Mangrove Ecosystem and Bird Sanctuary located in Kochi, Kerala at 9° 54′ N 76° 18′ E.

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Rajan, L., Chakraborty, K. & Chakraborty, R.D. Pharmacological properties of some mangrove sediment-associated bacillus isolates. Arch Microbiol 203, 67–76 (2021). https://doi.org/10.1007/s00203-020-01999-5

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  • DOI: https://doi.org/10.1007/s00203-020-01999-5

Keywords

  • Mangrove sediment-associated bacteria
  • Bacillus amyloliquefaciens MBMS5
  • Pharmacological properties
  • Antimicrobial
  • Antioxidant and anti-inflammatory activities