Methylobacterium planium sp. nov., isolated from a lichen sample

Jiang, Longqian; An, Defeng; Wang, Xinyu; Zhang, Kun; Li, Guiding; Lang, Lei; Wang, Lisong; Jiang, Chenglin; Jiang, Yi

doi:10.1007/s00203-020-01881-4

Original Paper
Published: 18 April 2020

Methylobacterium planium sp. nov., isolated from a lichen sample

Longqian Jiang¹,
Defeng An¹,
Xinyu Wang¹,
Kun Zhang¹,
Guiding Li^1,3,
Lei Lang¹,
Lisong Wang²,
Chenglin Jiang¹ &
Yi Jiang ORCID: orcid.org/0000-0002-1847-252X¹

Archives of Microbiology volume 202, pages 1709–1715 (2020)Cite this article

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Abstract

A novel bacterial strain, designated YIM 132548^T, was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China. The organism was Gram-stain negative, aerobic and methylotrophic. The cell was catalase positive and oxidase negative, asporogenous, rod-shaped and motile with three polar flagella. The strain could grow at 15–30 °C (optimum, 20 °C), at pH 6.0–9.0 (optimum, pH 7.0) and does not grow in the presence of NaCl. According to the 16S rRNA gene sequence analysis, strain YIM 132548^T showed high levels of 16S rRNA gene sequence similarity with Methylobacterium soli YIM 48816^T (97.6%) and Methylobacterium durans NBRC 112876^T (97.3%), less than 97.0% with other validly named type strains of the genus Methylobacterium. Ubiquinone Q-10 was the predominant respiratory ubiquinone. The predominant cellular fatty acid was identified as summed feature 8 (C_18:1ω7c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G + C content of the draft genome sequence is 70.2 mol%. The average nucleotide identity and digital DNA–DNA hybridizations values of strain YIM 132548^T with M. soli YIM 48816^T and M. durans NBRC 112876^T were 87.0% and 82.0%, 40.6% and 27.2% based on draft genome sequences, respectively. On the basis of phylogenetic, chemotaxonomic, phenotypic and genomic data, strain YIM 132548^T is concluded to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium planium sp. nov. is proposed. The type strain is YIM 132548^T (= CGMCC 1.17323^T = NBRC 114056^T).

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Abbreviations

DPG:: Diphosphatidylglycerol
PG:: Phosphatidylglycerol
PC:: Phosphatidylcholine
PME:: Phosphatidylmethylethanolamine
PE:: Phosphatidylethanolamine
AL:: Unidentified aminolipid
L:: Lipid
ANI:: Average nucleotide identity
dDDH:: Digital DNA–DNA hybridization

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Acknowledgements

This research was funded by National Natural Science Foundation of China (31460005) and Major research project of Guangxi for science and technology (AA18242026).

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Affiliations

Key Laboratory for Conservation and Utilization of Bio-Resource, School of Life Sciences, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China
Longqian Jiang, Defeng An, Xinyu Wang, Kun Zhang, Guiding Li, Lei Lang, Chenglin Jiang & Yi Jiang
Key Lab for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People’s Republic of China
Lisong Wang
Institute of Microbial Pharmaceuticals, Northeastern University, Shenyang, 110819, People’s Republic of China
Guiding Li

Authors

Longqian Jiang
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Defeng An
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Xinyu Wang
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Kun Zhang
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Guiding Li
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Lei Lang
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Lisong Wang
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Chenglin Jiang
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Yi Jiang
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Contributions

LJ performed the experiments and wrote the manuscript; XW collected the lichen samples; KZ and DFA analysed the data; GL and LL analysed the data; LW identified the lichen samples; YJ guided the experiments and revised the manuscript; CJ designed the study.

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Correspondence to Yi Jiang.

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The GenBank/EMBL/DDBJ Accession Number for the 16S rRNA gene sequence of strain YIM 132548^T is MN317338 and the genome sequence is VZZJ00000000.

Communicated by Erko Stackebrandt.

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Jiang, L., An, D., Wang, X. et al. Methylobacterium planium sp. nov., isolated from a lichen sample. Arch Microbiol 202, 1709–1715 (2020). https://doi.org/10.1007/s00203-020-01881-4

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Received: 25 December 2019
Revised: 22 March 2020
Accepted: 01 April 2020
Published: 18 April 2020
Issue Date: September 2020
DOI: https://doi.org/10.1007/s00203-020-01881-4

Keywords

Methylobacterium planium
New species
Lepraria sp. lichen