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Effect of changes at the conserved + 3 position of mature archaellins on in vitro cleavage by the pre-archaellin peptidase FlaK of Methanococcus maripaludis

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Abstract

Archaea swim using archaella that are domain-specific rotary type IV pilus-like appendages. The structural components of the archaellum filament are archaellins, initially made as preproteins with type IV pilin-like signal peptides which are removed by signal peptidases that are homologues of prepilin peptidases that remove signal peptides from type IV pilins. N-terminal sequences of archaellins, including the signal peptide cleavage site, are conserved and various positions have been previously shown to be critical for signal peptide removal. Archaellins have an absolute conservation of glycine at the + 3 position from the signal peptide cleavage site. To investigate its role in signal peptide cleavage, I used archaellin variants in which the + 3 glycine was mutated to all other possibilities in in vitro cleavage reactions. Cleavage was observed with ten different amino acids at the + 3 position, indicating that the observed glycine conservation is not required for this essential processing step.

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Acknowledgements

This research was supported by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (NSERC).

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  1. Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, ON, K7L 3N6, Canada

    Ken F. Jarrell

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  1. Ken F. Jarrell
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Correspondence to Ken F. Jarrell.

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Communicated by Michael Rother.

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Jarrell, K.F. Effect of changes at the conserved + 3 position of mature archaellins on in vitro cleavage by the pre-archaellin peptidase FlaK of Methanococcus maripaludis. Arch Microbiol 202, 1669–1675 (2020). https://doi.org/10.1007/s00203-020-01873-4

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