Skip to main content
SpringerLink
Log in

Potential application in amoxicillin removal of Alcaligenes sp. MMA and enzymatic studies through molecular docking

  • Original Paper
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Antibiotic contamination in environmental matrices is a serious global problem which leads to an increase in the proliferation of antibiotic resistance genes. Amoxicillin is ubiquitous in the environment, but there is hardly any information on the dissipation of amoxicillin by the microbial community. In view of this, the present study focusses on the removal of amoxicillin using amoxicillin-resistant bacteria, Alcaligenes sp. MMA. Bacteria were characterized using antibiotic tests, biochemical and molecular analysis. Alcaligenes sp. MMA was able to remove up to 84% of amoxicillin in 14 days in M9 minimal media, and the degradation products were confirmed using LC–MS/MS, including the benzothiazole, 2-Amino-3-methoxyl benzoic acid, 4-Hydroxy-2-methyl benzoic acid, 5-Amino-2-methylphenol and 3,5-Bis(tert-butyl)-2-hydroxybenzaldehyde, at the end of 14th day which further shows the removal of amoxicillin by the bacterial strain. Differential expression of porins was found in the presence of amoxicillin as a sole source of carbon and energy for the bacterial strain. Molecular interaction using in silico studies were performed which showed the formation of a hydrogen bond between amoxicillin and porins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig.1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Al Laham N, Chavda KD, Cienfuegos-Gallet AV, Kreiswirth BN, Chen L (2017) Genomic characterization of VIM metallo-β-lactamase-producing Alcaligenes faecalis from Gaza. Palestine Antimicrob Agents Chemother 61(11):e01499–e1517

    CAS  PubMed  Google Scholar 

  • Baquero F, Martínez JL, Cantón R (2008) Antibiotics and antibiotic resistance in water environments. Curr Opin Biotechnol 19(3):260–265

    Article  CAS  Google Scholar 

  • Coates ARM, Hu Y (2007) Novel approaches to developing new antibiotics for bacterial infections. Br J Pharmacol 152(8):1147–1154

    Article  CAS  Google Scholar 

  • Coyne MS, Coyne MS (1999) Soil microbiology: an exploratory approach. Delmar, New York

    Google Scholar 

  • Delcour AH (2009) Outer membrane permeability and antibiotic resistance. Biochimica et Biophysica Acta Proteins Proteomics 1794(5):808–816

    Article  CAS  Google Scholar 

  • Durán RE, Barra-Sanhueza B, Salvà-Serra F, Méndez V, Jaén-Luchoro D, Moore ER, Seeger M (2019) Complete genome sequence of the marine hydrocarbon degrader Alcaligenes aquatilis QD168, isolated from crude oil-polluted sediment of Quintero Bay Central Chile. Microbiol Resour Announc 8(5):e01664–e1718

    Article  Google Scholar 

  • Durso LM, Cook KL (2014) Impacts of antibiotic use in agriculture: what are the benefits and risks? Curr Opin Microbiol 19:37–44

    Article  Google Scholar 

  • Garrity A (2005) Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int J Syst Evol Microbiol 55:2235–2238

    Article  Google Scholar 

  • Holmes AH, Moore LS, Sundsfjord A, Steinbakk M, Regmi S, Karkey A, Guerin PJ, Piddock LJ (2016) Understanding the mechanisms and drivers of antimicrobial resistance. Lancet 387(10014):176–187

    Article  CAS  Google Scholar 

  • Hubicka U, Żmudzki P, Żuromska-Witek B, Zajdel P, Pawłowski M, Krzek J (2013) Separation and characterization of ciprofloxacin, difloxacin, lomefloxacin, norfloxacin, and ofloxacin oxidation products under potassium permanganate treatment in acidic medium by UPLC-MS/MS. Talanta 109:91–100

    Article  CAS  Google Scholar 

  • Konreddy AK, Rani GU, Lee K, Choi Y (2019) Recent drug-repurposing-driven advances in the discovery of novel antibiotics. Curr Med Chem 26(28):5363–5388

    Article  CAS  Google Scholar 

  • Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35(6):1547–1549

    Article  CAS  Google Scholar 

  • Kumar M, Jaiswal S, Sodhi KK, Shree P, Singh DK, Agrawal PK, Shukla P (2019) Antibiotics bioremediation: perspectives on its ecotoxicity and resistance. Environ Int 124:448–461

    Article  CAS  Google Scholar 

  • Laxminarayan R, Duse A, Wattal C, Zaidi AK, Wertheim HF, Sumpradit N, Vlieghe E, Hara GL, Gould IM, Goossens H, Greko C (2013) Antibiotic resistance—the need for global solutions. Lancet Infect Dis 13(12):1057–1098

    Article  Google Scholar 

  • Liang X, Chen B, Nie X, Shi Z, Huang X, Li X (2013) The distribution and partitioning of common antibiotics in water and sediment of the Pearl River Estuary South China. Chemosphere 92(11):1410–1416

    Article  CAS  Google Scholar 

  • Liu X, Huang D, Wu J, Yu C, Zhou R, Liu C, Zhang W, Yao J, Cheng M, Guo S (2016) The genome sequence of Alcaligenes faecalis NBIB-017 contains genes with potentially high activities against Erwinia carotovora. Genome Announc 4(2):e00222–e316

    PubMed  PubMed Central  Google Scholar 

  • Numminen I (2019) The occurrence and problems of residual antibiotics during wastewater treatment.

  • Quiroz-Castañeda RE, Mendoza-Mejía A, Obregón-Barboza V, Martínez-Ocampo F, Hernández-Mendoza A, Martínez-Garduño F, Guillén-Solís G, Sánchez-Rodríguez F, Peña-Chora G, Ortíz-Hernández L, Gaytán-Colín P (2015) Identification of a new Alcaligenes faecalis strain MOR02 and assessment of its toxicity and pathogenicity to insects. BioMed Res Int 2015.

  • Regar RK, Gaur VK, Mishra G, Jadhao S, Kamthan M, Manickam N (2016) Draft genome sequence of Alcaligenes faecalis strain IITR89, an indole-oxidizing bacterium. Genome Announc 4(2):e00067-16

    PubMed  PubMed Central  Google Scholar 

  • Sugawara E, Kojima S, Nikaido H (2016) Klebsiella pneumoniae major porins OmpK35 and OmpK36 allow more efficient diffusion of β-lactams than their Escherichia coli homologs OmpF and OmpC. J Bacteriol 198(23):3200–3208

    Article  CAS  Google Scholar 

  • Sodhi KK, Kumar M, Balan B, Dhaulaniya AS, Singh DK (2020) Isolation and characterization of amoxicillin-resistant bacteria and amoxicillin-induced alteration in its protein profiling and RNA yield. Arch Microbiol 202(2):225–232

    Article  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24(8):1596–1599

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Financial assistance is provided by NASF research grant (project entitled ‘Bioremediation of chemical contaminants and their complexes present in drainage water with high dynamic flux used for irrigation in urban and peri urban agriculture’), sanction no. NASF/CA-6030/2017–18 is highly acknowledged. The authors Kushneet Kaur Sodhi and Mohit Kumar highly acknowledges the University Grant Commission (UGC), Government of India for providing the stipend.

Funding

This study was supported by the funding agency, National Agricultural Science Fund, Indian Council of Agricultural Research, Delhi, India.

Author information

Author notes

  1. Kushneet Kaur Sodhi and Mohit Kumar both are equal authors.

Authors and Affiliations

  1. Soil Microbial Ecology and Environmental Toxicology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007, India

    Kushneet Kaur Sodhi, Mohit Kumar & Dileep Kumar Singh

Authors
  1. Kushneet Kaur Sodhi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mohit Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dileep Kumar Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dileep Kumar Singh.

Ethics declarations

Conflict of interest

No conflicts of interest.

Research involving human participants and/or animals

This study does not related with animals or humans.

Additional information

Communicated by Erko Stackebrandt.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sodhi, K.K., Kumar, M. & Singh, D.K. Potential application in amoxicillin removal of Alcaligenes sp. MMA and enzymatic studies through molecular docking. Arch Microbiol 202, 1489–1495 (2020). https://doi.org/10.1007/s00203-020-01868-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-020-01868-1

Keywords

Search

Navigation