Abstract
An aerobic bacterium, designated strain Dysh456T, was isolated from a crude oil-contaminated soil. Cells of strain Dysh456T were rod-shaped, motile, and Gram-stain-negative. Strain Dysh456T grew at 13–48 °C and pH 4.3–7.9. Major cellular fatty acids were iso-C15:0 (42.5%), iso-C17:0 (15.3%) and summed feature 9 (iso-C17:1 ω9c/C16:0 10-methyl [13.7%]). Major respiratory quinone was ubiquinone-8. The genome of strain Dysh456T consists of a single circular chromosome of 2,874,969 bp in length with G + C content of 68.3%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain Dysh456T belongs to the family Rhodanobacteraceae, but none of the existing genera can accommodate this novel isolate. On the basis of physiological, chemotaxonomic, and genomic properties, strain Dysh456T (= NBRC 112897T = DSM 105662T) is proposed as the type strain representing a novel species of novel genus, for which the name Aerosticca soli gen. nov., sp. nov. is proposed.
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Acknowledgements
Authors thank R. Tokizawa, Hokkaido University, for her technical assistance. We also thank Dr. Y. Higashioka, National Institute of Technology, Kochi College, for her assistance in soil sampling. The funding was received by Japan Society for the Promotion of Science (Grant no. 22370005) and Research Fellow of Japan Society for the Promotion Science.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence and the complete genome sequence of the strain Dysh456T are LC276682 and AP018560, respectively.
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Watanabe, M., Kojima, H. & Fukui, M. Aerosticca soli gen. nov., sp. nov., an aerobic gammaproteobacterium isolated from crude oil-contaminated soil. Arch Microbiol 202, 1069–1076 (2020). https://doi.org/10.1007/s00203-020-01819-w
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DOI: https://doi.org/10.1007/s00203-020-01819-w