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Alterations of salivary microbial community associated with oropharyngeal and hypopharyngeal squamous cell carcinoma patients

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Abstract

The highest number (35.1% of global incident cases) of new oropharyngeal (OP) and hypopharyngeal (HP) cancer cases was reported in South-Central Asia. The highest incidence of HP cancer in India was reported in East Khasi Hills District of Meghalaya, Aizawl District of Mizoram, and Kamrup Urban District of Assam. HP and OP cancer showed the highest mortality rate, worst prognoses and the highest rate of nodal metastases and distant metastases. Thus, research is required to detect specific biomarkers for early prevention and diagnosis for these cancers. Oral microbiome signatures in saliva are considered as a potential diagnostic biomarker for OP and HP cancer. Bacterial profile alterations in OP and HP cancer have not been reported in India population, to establish the association of oral bacteria in the progression of OP and HP cancer; we studied bacterial communities in saliva of eight OP and seven HP cancer patients as compared to healthy controls using 16S rRNA V3–V4 region sequencing. The higher abundance of Haemophilus parainfluenzae, Haemophilus influenzae and Prevotella copri and lower abundance of Rothia mucilaginosa, Aggregatibacter segnis, Veillonella dispar, Prevotella nanceiensis, Rothia aeria, Capnocytophaga ochracea, Neisseria bacilliformis, Prevotella nigrescens and Selenomonas noxia in saliva of OP and HP cancer patients may be considered as a non-invasive diagnostic biomarker for OP and HP cancer patients. Streptococcus anginosus may be considered as a non-invasive diagnostic biomarker for OP cancer patients only. Therefore, evaluation of salivary microbial biomarkers may be informative to understand the pathobiology and carcinogenesis of OP and HP cancer.

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Acknowledgements

The authors are thankful to Genotypic Technology Pvt Ltd, Bangalore, India for providing technical support at metagenomic services. The authors are thankful to NER-BPMC, Department of Biotechnology, New Delhi, India for funding the research grant (Grant No. BT/PR16886/NER/95/336/2015). The authors are thankful to Indian Council of Medical Research for providing senior research fellowship to Mrs. Madhusmita Panda.

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Author notes

  1. Madhusmita Panda and Avdhesh Kumar Rai have contributed equally to this work.

Authors and Affiliations

  1. Department of Life Sciences, School of Life Sciences, Central University of Tamil Nadu, Thiruvarur, 610005, India

    Madhusmita Panda & Indranil Chattopadhyay

  2. Dr. B. Borooah Cancer Institute, A. K. Azad Road, Gopinath Nagar, Guwahati, Assam, 781016, India

    Avdhesh Kumar Rai, Tashnin Rahman, Ashok Das, Rajjyoti Das, Anupam Sarma & Amal Ch. Kataki

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  1. Madhusmita Panda
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Panda, M., Rai, A.K., Rahman, T. et al. Alterations of salivary microbial community associated with oropharyngeal and hypopharyngeal squamous cell carcinoma patients. Arch Microbiol 202, 785–805 (2020). https://doi.org/10.1007/s00203-019-01790-1

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