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Acinetobacter pullicarnis sp. nov. isolated from chicken meat

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Abstract

A novel bacterial strain, named S23T, was isolated from chicken meat of local market in Korea. Cells were Gram-negative, milky-yellow colored, non-motile and coccobacillus. The strain was obligate aerobic and catalase-positive, oxidase-negative, optimum growth temperature and pH were 25 °C and pH 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain S23T belongs to the genus Acinetobacter and is most closely related to Acinetobacter defluvii KCTC 52503 T (97.40%). The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) value between strain S23T and its closet phylogenetic neighbors was below 76% and 17%, respectively. The G + C content of genomic DNA of strain S23T was 41.53 mol%. The major respiratory quinone was Q-9. The major cellular fatty acids were summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c), C18:1ω9c, and C16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanol-amine, and phosphatidylserine. The ANI and dDDH results and results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain S23T represented a novel species within the genus Acinetobacter, for which the name Acinetobacter pullicarnis sp. nov. is proposed. The strain type is S23T (= KACC 19921 T = JCM 33150 T).

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Abbreviations

ANI:

Average nucleotide identity

TEM:

Transmission electron microscopy

TSA:

Tryptic soy agar

MA:

MacConkey agar

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

PE:

Phosphatidylethanol-amine

PS:

Phosphatidylserine

APL:

Aminophospholipid

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015R1A2A2A01003993). This research was also supported by the Chung-Ang University Graduate Research Scholarship in 2017.

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  1. Department of Animal Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea

    Rae-Hee Han, Ju-Eun Lee, Sung-Hee Yoon & Geun-Bae Kim

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  1. Rae-Hee Han
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  2. Ju-Eun Lee
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  4. Geun-Bae Kim
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Correspondence to Geun-Bae Kim.

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Han, RH., Lee, JE., Yoon, SH. et al. Acinetobacter pullicarnis sp. nov. isolated from chicken meat. Arch Microbiol 202, 727–732 (2020). https://doi.org/10.1007/s00203-019-01785-y

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