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Bradyrhizobium sp. enhance ureide metabolism increasing peanuts yield

Archives of Microbiology volume 202, pages 645–656 (2020)Cite this article

Abstract

This study aimed to evaluate the effects of seed inoculation with Bradyrhizobium sp. and co-inoculation with Azospirillum brasilense. The seed treatments were as follows: control (without inoculation); A. brasilense (2 mL per kg−1 of seed); A. brasilense (4 mL per kg−1 of seed); Bradyrhizobium sp. (2 mL per kg−1 of seed); Bradyrhizobium sp. (4 mL per kg−1 of seed); A. brasilense + Bradyrhizobium sp. (2 mL of each strain per kg−1 of seed); and A. brasilense + Bradyrhizobium sp. (4 mL of each strain per kg−1 of seed). Peanut plants from seeds inoculated with Bradyrhizobium sp. and A. brasilense exhibited highest leaf concentration of photosynthetic pigments, carotenoids, nitrate, ammonia and amino acids. The inoculation of seeds with Bradyrhizobium sp. resulted in plants with increased concentrations of total soluble sugars, and ureides compared to the untreated plants. In contrast, seeds treated with A. brasilense alone resulted in plants exhibiting highest concentration of amino acids, which represent the highest concentration of nitrogen compounds in peanut plants. Seed inoculation with Bradyrhizobium sp. at a rate of 2 mL kg−1 was identified as the best treatment to promote increased biological nitrogen fixation and generate higher peanut yields.

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Acknowledgements

The first author thanks the Brazilian Federal Agency for the Support and Evaluation of Graduate Education (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; CAPES) for the scholarship. ARR thanks the National Council for Scientific and Technological Development (CNPq) for the research fellowship (Grant number 309380/2017-0). The authors also thank Agrocete Indústria de Fertilizantes Ltda, for providing the inoculants GRAP NOD am (Bradyrhizobium sp., SEMIA 6144) and GRAP NOD a (Azospirillum brasilense, strains AbV5 and AbV6).

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Authors and Affiliations

  1. São Paulo State University (UNESP), Tupã, SP, 17602-496, Brazil

    Thais Grassi Gericó, Sandra Cristina de Oliveira, Ana Elisa Bressan Smith Lourenzani & André Rodrigues dos Reis

  2. São Paulo State University (UNESP), Ilha Solteira, SP, 15385-000, Brazil

    Renan Francisco Rimoldi Tavanti & André Rodrigues dos Reis

  3. São Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil

    Jéssica Pacheco de Lima

  4. Beatrice Comércio Importação Exportação Amendoim Ltda, Tupã, SP, 17607-020, Brazil

    Rodolfo Pires Ribeiro

  5. El Carmen Sementes Do Brasil, Tupã, SP, 17607-020, Brazil

    Leandro Cesar Cusim dos Santos

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  1. Thais Grassi Gericó
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  2. Renan Francisco Rimoldi Tavanti
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Correspondence to André Rodrigues dos Reis.

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Gericó, T.G., Tavanti, R.F.R., de Oliveira, S.C. et al. Bradyrhizobium sp. enhance ureide metabolism increasing peanuts yield. Arch Microbiol 202, 645–656 (2020). https://doi.org/10.1007/s00203-019-01778-x

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  • DOI: https://doi.org/10.1007/s00203-019-01778-x

Keywords

  • Arachis hypogaea L.
  • Growth-promoting bacteria
  • Nitrogen metabolism
  • Seed inoculation
  • Photosynthetic pigments