Abstract
The structure and diversity of microbial communities in the leaves of Cinnamomum camphora at different growth stages were studied by high-throughput sequencing. Moreover, the relationships between microbial communities and borneol content were analyzed in this paper. The results indicated that the community structure of endophytic bacteria in C. camphora exhibited temporal variations, with the microbial diversity presented as follows: T1 (low content period) > T3 (peak period) > T2 (small peak period). The population of endophytic bacteria and the ratio of primary metabolism in the leaves of C. camphora were T2 > T1 > T3, while the metabolic intensity of endophytic bacterial terpenoids and polyketides was T3 > T2 > T1, which had the same trend as borneol content in C. camphora. The metabolic ratio of terpenoids and polyketides in T3 was 7.44% higher than that in T1, while that in T2 was 4.10% higher than that in T1. The abundance and diversity of Clostridium_sensu_stricto_1, Ochrobactrum, Escherichia-Shigella, Pseudomonas, and Massilia significantly promoted the content of terpenoids in C. camphora. Together, those results provide the first evidence that borneol content and potential metabolic intensity in leaves of C. camphora greatly depend on microbial communities composition and diversity.
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Acknowledgements
We are grateful to Professor Huihua Fan of the Fujian Academy of Forestry for his valuable comments and suggestions on this manuscript. We are also grateful to Shengen Liu of the Institute of Applied Ecology, Chinese Academy of Science for his useful assistance in data analyzing.This work was supported by the research on Cinnamomum camphora Excellent Germplasm Selection and Rapid Propagation Technology (2014R1011-4).
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Zhang, Gf., Huang, Ql., Bi, Xq. et al. Analysis of endophytic bacterial community diversity and metabolic correlation in Cinnamomum camphora. Arch Microbiol 202, 181–189 (2020). https://doi.org/10.1007/s00203-019-01733-w
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DOI: https://doi.org/10.1007/s00203-019-01733-w