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Characterization and biotechnological application of protease from thermophilic Thermomonas haemolytica

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Abstract

In this study, it was aimed to determine the ability to produce protease enzyme of Thermomonas haemolytica isolated from geothermal Nenehatun hot spring in Turkey and utilization of this enzyme in the detergent industry to remove protein stains. The protease-producing strains were screened from hot springs, and a potential strain was identified as T. haemolytica according to morphological, physiological and biochemical characteristics and sequence of 16S rRNA gene. Maximum protease activity was observed at 55 °C and pH 9.0 at 72 h of incubation. Activity was very stable between 50 and 65 °C and pH 8.0–10.0, respectively. The enzyme activity was significantly inhibited by PMSF and partly inhibited by EDTA, EGTA, SDS, and urea. Some divalent metal ions such as Ca2+, Mg2+, and Mn2+ increased the enzyme activity, while Zn2+ and Cu2+ decreased. Michaelis–Menten constant (Km) and maximum velocity (Vmax) values were calculated by Lineweaver–Burk plot as 125 EU/ml and 1262 mg/ml, respectively. The biochemical characterization of the protease obtained from T. haemolytica was performed and applied on the blood and grass-stained fabrics with detergent to evaluate the stain removal performance of the enzyme. It was observed that the application of detergent with enzyme was more effective than the detergent without enzyme to clean up the stained fabrics. This is the first report of characterization of the protease of T. haemolytica. According to results obtained from this study, this new strain is a promising candidate for industrial applications in production of detergent.

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Acknowledgements

The authors would like to thank the officials of the Erzurum Technical University High-Technology Applications and Research Center (YUTAM) for their precious contribution and also the municipal officials who helped in obtaining the water samples from the Erzurum hot springs.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey

    Ebru Oztas Gulmus & Arzu Gormez

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  1. Ebru Oztas Gulmus
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  2. Arzu Gormez
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Correspondence to Arzu Gormez.

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Communicated by Erko Stackebrandt.

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Oztas Gulmus, E., Gormez, A. Characterization and biotechnological application of protease from thermophilic Thermomonas haemolytica. Arch Microbiol 202, 153–159 (2020). https://doi.org/10.1007/s00203-019-01728-7

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