Abstract
To evaluate the changes in bacterial diversity at various time points under resveratrol supplementation, we aimed to investigate the diversification of gut microbiota and the changes in total genetic diversity. We performed 16S rDNA gene sequencing at different time points (15, 30, and 45 days) to analyze the gut microbiota of tilapia. Fusobacteria, Proteobacteria, and Bacteroidetes (15 days) or Cyanobacteria (30 and 45 days) were found to be the three most abundant phyla. Cyanobacteria (15 and 30 days), Proteobacteria (15 days), Firmicutes and Chlamydiae (30 and 45 days), Planctomycetes (30 days), Bacteroidetes, Actinobacteria, and Fusobacteria (45 days) in the 0.05 g/kg RES group increased as compared to that in the controls. Proteobacteria and Cyanobacteria significantly decreased and increased at 30 and 45 days, respectively, while the reverse pattern was observed at 15 days. The Bacteroidetes:Firmicutes and Proteobacteria:Cyanobacteria ratios were significantly increased (15 and 45 days, P < 0.05) and decreased (30 days, P < 0.05). RES supplementation did not affect the richness and diversity of the gut microbiota in tilapia. Our findings may contribute to the development of strategies for the management of diseases.
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Genetic data have been deposited in the NCBI Short Read Archive (SRA) under the project accession PRJNA392982. We thank Gihanna Gaye for providing grammar and spelling check of the manuscript. The work was supported by Special Fund of Fundamental Scientific Research Business Expense for Central Public Research Institutes (No. 2019JBFZ06), National Science and Technology Pillar Program (2015BAD13B03), China Agriculture Research System (No. CARS-46).
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YZ, XWB, JZC conceived and designed the experiments; YZ analyzed the data; LPQ, SLM, CS, WW, GDH contributed reagents/materials/analysis tools; YZ contributed to figures preparation; YZ prepared and wrote the manuscript. All authors reviewed the manuscript.
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Zheng, Y., Hu, G., Wu, W. et al. Time-dependent gut microbiota analysis of juvenile Oreochromis niloticus by dietary supplementation of resveratrol. Arch Microbiol 202, 43–53 (2020). https://doi.org/10.1007/s00203-019-01712-1
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DOI: https://doi.org/10.1007/s00203-019-01712-1