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Amniculibacterium aquaticum gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a stream

Abstract

Strain KYPW7T, isolated from the Funglin Stream in Taiwan, was characterized using a polyphasic taxonomy approach. Cells of strain KYPW7T were Gram-stain-negative, aerobic, non-spore forming, non-motile rods and formed white colonies. Growth occurred at 15–30 °C (optimum 25 °C), at pH 6–8 (optimum pH 6.5) and with 0–1% NaCl (optimum 0%). Phylogenetic analyses based on 16S rRNA and coding sequences of 92 protein clusters showed that strain KYPW7T represents a novel genus in the family Flavobacteriaceae. The 16S rRNA gene sequence of strain KYPW7T was related to the species of the genera Chryseobacterium (91.8–96.0% sequence similarity), Bergeyella (95.1–95.8%), Cloacibacterium (94.5–95.7%), Daejeonia (95.6%) and Riemerella (94.0–95.0%). Strain KYPW7T showed less than 72% average nucleotide identity and less than 24% digital DNA–DNA hybridization identity compared to the type strains of related genera within the family Flavobacteriaceae. The predominant fatty acids were iso-C15:0 and iso-C17:0 3-OH. The major isoprenoid quinone was MK-6 and the DNA G + C content was 36.8 mol%. The polar lipids had phosphatidylethanolamine, three uncharacterized aminophospholipids and an uncharacterized phospholipid. The polyamines contained homospermidine, putrescine and spermidine. On the basis of the genotypic and phenotypic data, strain KYPW7T represents a novel species of a new genus in the family Flavobacteriaceae, for which the name Amniculibacterium aquaticum gen. nov., sp. nov. is proposed. The type strain is KYPW7T (= BCRC 81123T = LMG 30598T = KCTC 62512T).

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Communicated by Erko Stackebrandt.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and the draft genome of Amniculibacterium aquaticum strain KYPW7T are MG603666 and REFA00000000. The Digital Protologue database Taxon Numbers for genus Amniculibacterium and strain KYPW7T are GA00107 and TA00829, respectively.

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Chen, WM., Guo, YP., Sheu, C. et al. Amniculibacterium aquaticum gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a stream. Arch Microbiol 201, 1119–1127 (2019). https://doi.org/10.1007/s00203-019-01688-y

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Keywords

  • Amniculibacterium aquaticum gen. nov., sp. nov.
  • Bacteroidetes
  • Flavobacteriia
  • Flavobacteriales
  • Flavobacteriaceae
  • Polyphasic taxonomy