Abstract
Bovine tuberculosis is a disease that is widely distributed around the world. Its causative agent, Mycobacterium bovis, has characteristics of a microorganism with clonal multiplication in populations with no evidence of genetic exchange between strains, and, consequently, a group of strains can be identified as descending from a common ancestor. The aim of this study was to investigate the clonal complexes of M. bovis isolated from samples of lesions suggestive of bovine tuberculosis collected from slaughterhouses in various states of Brazil between 2006 and 2012. Ninety samples were analyzed, and it was found that 14.4% belonged to the clonal complex European1 and 81.1% to the clonal complex European2, while 4.65% were not identified as any of the four known complexes.
Similar content being viewed by others
References
Araujo CP, Osorio ALAR, Jorge KSG, Ramos CAN, Souza Filho AF, Vidal CES et al (2014) Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR. Braz J Microbiol 45(2):633–640
Berg S, Garcia-Pelayo MC, Mueller B, Hailu E, Asiimwe B, Kremer K et al (2011) African 2, a clonal complex of Mycobacterium bovis epidemiologically important in East Africa. J Bacteriol 193(3):670–678. https://doi.org/10.1128/jb.00750-10
Canevari Castelão AB, Nishibe C, Moura A, de Alencar AP, de Azevedo Issa M, Hodon MA et al (2014) Draft genome sequence of Mycobacterium bovis strain AN5, used for production of purified protein derivative. Genome Announc 2(2):e00277-14. https://doi.org/10.1128/genomeA.00277-14
Cardoso MA, Cardoso RF, Hirata RDC, Hirata MH, Leite CQF, Santos ACB et al (2009) Direct detection of Mycobacterium bovis in bovine lymph nodes by PCR. Zoonoses Public Health 56(8):465–470. https://doi.org/10.1111/j.1863-2378.2008.01199.x
Comas I, Gagneux S (2009) The past and future of tuberculosis research. PLoS Pathog 5(10):e1000600. https://doi.org/10.1371/journal.ppat.1000600
Dani MA, Heinneman MB, Dani SU (2008) Brazilian Nelore cattle: a melting pot unfolded by molecular genetics. Genet Mol Res 7(4):1127–1137
de Souza Figueiredo EE, Silvestre FG, Campos WN, Furlanetto LV, Medeiros L, Lilenbaum W et al (2009) Identification of Mycobacterium bovis isolates by a multiplex PCR. Braz J Microbiol 40(2):231–233
Fitzgerald SD, Kaneene JP (2013) Wildlife reservoirs of bovine tuberculosis worldwide: hosts, pathology, surveillance, and control. Vet Pathol 50(3):488–499. https://doi.org/10.1177/0300985812467472
Lasserre M, Fresia P, Greif G, Iraola G, Castro-Ramos M, Juambeltz A, Nuñez Á, Naya H, Robello C, Berná L (2018) Whole genome sequencing of the monomorphic pathogen Mycobacterium tuberculosis reveals local differentiation of cattle clinical isolates. BMC Genom 19(1):2. https://doi.org/10.1186/s12864-017-4249-6
Lilenbaum W, Marassi CD, Medeiros LS (2010) Controlling bovine TB. Vet Rec 167(17):669–670. https://doi.org/10.1136/vr.c5885
Müller B, Hilty M, Berg S, Garcia-Pelayo MC, Dale J, Boschiroli ML et al (2009) African 1, an epidemiologically important clonal complex of Mycobacterium bovis dominant in Mali, Nigeria, Cameroon, and Chad. J Bacteriol 191(6):1951–1960. https://doi.org/10.1128/JB.01590-08
Orloski K, Robbe-Austerman S, Stuber T, Hench B, Schoenbaum M (2018) Whole genome sequencing of Mycobacterium bovis isolated from livestock in the United States, 1989–2018. Front Vet Sci 5:253
Parreiras PM, Andrade GI, do Nascimento TDF, Oelemann MC, Gomes HM, de Alencar AP et al (2012) Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil. Mem Inst Oswaldo Cruz 107(1):64–73
Ramos DF, Tavares L, da Silva PE, Dellagostin AO (2014a) Molecular typing of Mycobacterium bovis isolates: a review. Braz J Microbiol 45(2):365–372
Ramos DF, Silva AB, Fagundes MQ, von Groll A, da Silva PE, Dellagostin AO (2014b) Molecular typing of Mycobacterium bovis isolated in the south of Brazil. Braz J Microbiol 45(2):657–660
Rocha VC, de Figueiredo SC, Rosales CA, de Hildebrand e Grisi Filho JH, Keid LB, Soares RM et al (2013) Molecular discrimination of Mycobacterium bovis in São Paulo, Brazil. Vector Borne Zoonotic Dis 13(1):17–21. https://doi.org/10.1089/vbz.2012.1035
Rodriguez-Campos S, Schurch AC, Dale J, Lohan AJ, Cunha MV, Botelho A et al (2012) European 2—a clonal complex of Mycobacterium bovis dominant in the Iberian Peninsula. Infect Genet Evol 12(4):866–872. https://doi.org/10.1016/j.meegid.2011.09.004
Sales ML, Fonseca AA, Sales EB, Cottorello AC, Issa MA, Hodon MA et al (2014a) Evaluation of molecular markers for the diagnosis of Mycobacterium bovis. Folia Microbiol (Praha) 59(5):433–438. https://doi.org/10.1007/s12223-014-0317-3
Sales ML, Fonseca AA, Orzil L, Alencar AP, Hodon MA, Issa MA et al (2014b) Validation of two real-time PCRs targeting the PE-PGRS 20 gene and the region of difference 4 for the characterization of Mycobacterium bovis isolates. Genet Mol Res 13(2):4607–4616. https://doi.org/10.4238/2014.June.18.3
Sales ML, Fonseca Júnior AA, Orzil L, Alencar AP, Silva MR, Issa MA et al (2014c) Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis. Braz J Microbiol 45(4):1363–1369
Sanou A, Tarnagda Z, Kanyala E, Zingué D, Nouctara M, Ganamé Z et al (2014) Mycobacterium bovis in Burkina Faso: epidemiologic and genetic links between human and cattle isolates. PLoS Negl Trop Dis 8(10):e3142. https://doi.org/10.1371/journal.pntd.0003142
Smith NH (2012) The global distribution and phylogeography of Mycobacterium bovis clonal complexes. Infect Genet Evol 12(4):857–865. https://doi.org/10.1016/j.meegid.2011.09.007
Smith NH, Dale J, Inwald J, Palmer S, Gordon SV, Hewinson RG et al (2003) The population structure of Mycobacterium bovis in Great Britain: clonal expansion. Proc Natl Acad Sci USA 100(25):15271–15275. https://doi.org/10.1073/pnas.2036554100
Smith NH, Gordon SV, de la Rua-Domenech R, Clifton-Hadley RS (2006) Hewinson RG (2006) Bottlenecks and broomsticks: the molecular evolution of Mycobacterium bovis. Nat Rev Microbiol 4(9):670–681. https://doi.org/10.1038/rnmicro1472
Smith NH, Berg S, Dale J, Allen A, Rodriguez S, Romero B et al (2011) European 1: a globally important clonal complex of Mycobacterium bovis. Infect Genet Evol 11(6):1340–1351. https://doi.org/10.1016/j.meegid.2011.04.027
Zanini MS, Moreira EC, Salas CE, Lopes MTP, Barouni AS, Roxo E et al (2005) Molecular typing of Mycobacterium bovis isolates from south-east Brazil by spoligotyping and RFLP. J Vet Med Ser B Infect Dis Vet Public Health. https://doi.org/10.1111/j.1439-0450.2005.00835.x
Zimpel CK, Brandão PE, de Souza Filho AF, de Souza RF, Ikuta CY, Ferreira Neto JS et al (2017) Complete genome sequencing of Mycobacterium bovis SP38 and comparative genomics of Mycobacterium bovis and M. tuberculosis strains. Front Microbiol 8:2389. https://doi.org/10.3389/fmicb.2017.02389
Acknowledgements
We thank Dr. Ricardo Augusto Dias (VPS/FMVZ/USP) for his help in drawing up the map.
Funding
Funding was provided by Núcleo de Estudos, Pesquisa e Assessoria à Saúde and Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by Erko Stackebrandt.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Sales, É.B., de Alencar, A.P., Hodon, M.A. et al. Identification of clonal complexes of Mycobacterium bovis in Brazil. Arch Microbiol 201, 1047–1051 (2019). https://doi.org/10.1007/s00203-019-01674-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00203-019-01674-4