Characterization of Labrenzia polysiphoniae sp. nov. isolated from red alga Polysiphonia sp.

Abstract

A group of five Gram-negative aerobic halophilic bacteria was isolated from the red alga Polysiphonia sp. specimen collected from the Sea of Japan seashore and subjected to a taxonomic study. On the basis of 16S rRNA gene sequence analysis, the novel isolates were affiliated to the genus Labrenzia sharing the highest gene sequence similarities of 98.1–98.4% with the type strain of Labrenzia suaedae KACC 13772T. The DNA–DNA hybridization values of 83–91% obtained between five novel strains, and 26 and 36% between two of the five novel strains and the closest neighbor Labrenzia suaedae KACC 13772T confirmed their assignment to the same separate species. Novel isolates were characterized by Q-10 as the major ubiquinone, by the predominance of C18:1ω7c followed by 11-methyl C18:1ω7c and C14:0 3-ОН in their fatty acid profiles. Polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, an unknown aminophospholipid, and an unknown phospholipid. Some of novel strains were found to inhibit growth of Gram-negative and Gram-positive test microorganisms. On the basis of phylogenetic analysis, DNA–DNA hybridization and phenotypic traits, a novel species with the name Labrenzia polysiphoniae sp. nov. (type strain KMM 9699T = rh46T = KACC 19711T), is proposed.

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Acknowledgements

We would like to thank Dr. Soon-Wo Kwon, Korean Agricultural Culture Collection, KACC, Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Republic of Korea, for providing the type strain Labrenzia suaedae KACC 13772T for comparative analyses. This study was supported by a programme of “Bioresource collections”.

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Correspondence to Lyudmila A. Romanenko.

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The DDBJ/GenBank/EMBL accession number of the 16S rRNA gene sequence of strain KMM 9699T is LC381921.

Communicated by Erko Stackebrandt.

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Romanenko, L.A., Kurilenko, V.V., Guzev, K.V. et al. Characterization of Labrenzia polysiphoniae sp. nov. isolated from red alga Polysiphonia sp.. Arch Microbiol 201, 705–712 (2019). https://doi.org/10.1007/s00203-019-01640-0

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Keywords

  • Labrenzia polysiphoniae sp. nov.
  • Marine
  • Red alga
  • Polysiphonia sp.