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Williamsia aurantiacus sp. nov. a novel actinobacterium producer of antimicrobial compounds isolated from the marine sponge

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Abstract

An antibiotic-producing actinobacterium, designated isolate B375T, was isolated from marine sponge Glodia corticostylifera collected from Praia Guaecá, São Paulo, Brazil (23°49S; 45°25W), and its taxonomic position established using data from a polyphasic study. The organism showed a combination of morphological, physiological, biochemical and chemotaxonomic characteristics consistent with its classification in the genus Williamsia. Comparative 16S rRNA gene sequence analysis indicated that the strain B375T was most closely related to Williamsia serinedens DSM 45037T and Williamsia spongiae DSM 46676T and having 99.43% and 98.65% similarities, respectively, but was distinguished from these strains by a low level of DNA–DNA relatedness (53.2–63.2%) and discriminatory phenotypic properties. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and N-glycolated muramic acid residues present in the wall cells. The cells contained C16:0 (23.3%), C18:0 10-methyl (23.2%) and C18:1 ω9c (21.6%) as the major cellular fatty acids. The strain B375T inhibited growing of Staphylococcus aureus and Colletotrichum gloeosporioides strains and was considered a producer of antimicrobial compounds. Based on the data obtained, the isolate B375T (= CBMAI 1090T = DSM 46677T) should, therefore, be classified as the type strain of a novel species of the genus Williamsia, for which the name Williamsia aurantiacus sp. nov. is proposed.

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Acknowledgements

We thank Roberto Gomes de Souza Berlinck at the Universidade de São Paulo, São Carlos, Brazil, by collection from marine macroorganisms.

Funding

This research was kindly supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; Grants 2009/53320-7, 2011/50243-1 and 2016/22023-0).

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Authors and Affiliations

  1. Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, UNICAMP, Av. Alexandre Cazelatto 999, Paulínia, SP, CEP 13148-218, Brazil

    Cláudia Beatriz Afonso de Menezes, Rafael Sanches Afonso & Fabiana Fantinatti-Garboggini

  2. Programa de Pós-Graduação em Genética e Biologia Molecular, UNICAMP, CP 6109, Campinas, SP, 13083-970, Brazil

    Cláudia Beatriz Afonso de Menezes & Fabiana Fantinatti-Garboggini

  3. Embrapa Meio Ambiente, Rodovia SP 340 Km 127.5, CP 69, Jaguariúna, SP, CEP 13820-000, Brazil

    Wallace Rafael de Souza, Márcia Maria Parma & Itamar Soares de Melo

  4. Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Av. Bandeirantes,3900, Monte Alegre, Ribeirão Preto, São Paulo, 12040-901, Brazil

    Fernando Lucas Satoru Fugita & Luiz Alberto Beraldo Moraes

  5. Agrivalle, Av. Tranquillo Giannini, 1090, Salto, SP, 13329-600, Brazil

    Tiago Domingues Zucchi

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  1. Cláudia Beatriz Afonso de Menezes
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  9. Fabiana Fantinatti-Garboggini
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Correspondence to Fabiana Fantinatti-Garboggini.

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Additional information

Communicated by Erko Stackebrandt.

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The GenBank accession number for the 16S rRNA sequence of Williamsia aurantiacus B375 (CBMAI 1090T = DSM 46677T) is JN615438. The DPD Taxon Number is TA00751.

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de Menezes, C.B.A., Afonso, R.S., de Souza, W.R. et al. Williamsia aurantiacus sp. nov. a novel actinobacterium producer of antimicrobial compounds isolated from the marine sponge. Arch Microbiol 201, 691–698 (2019). https://doi.org/10.1007/s00203-019-01633-z

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