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Flavobacterium edaphi sp. nov., isolated from soil from Jeju Island, Korea

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Abstract

An aerobic, Gram-stain-negative, bright yellow-pigmented, oxidase and catalase-positive, non-motile, non-spore forming, rod-shaped strain designated DMN11T was isolated from the soil of crossroads of Jeju Island in South Korea. Colonies were circular, bright yellow-pigmented and smooth with regular edges and measured approximately 1–2 mm in diameter. Flexirubin-type pigments were absent. Phylogenetic tree analysis based on the 16SrRNA gene sequence revealed that the strain DMN11T formed a lineage within the family Flavobacteriaceae of the phylum Bacteroidetes, and it was the most closely related to Flavobacterium suzhouense XIN-1T and Flavobacterium hauense BX12T (98.6% and 98.2% similarity, respectively). The major isoprenoid quinone was MK-6. The major fatty acids were summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c), iso-C15:0 and iso-C15:0 3OH. The polar lipid profile of the strain DMN11T showed the presence of phosphatidylethanolamine (PE) as major lipid. The DNA G+C content was 35.3 mol%, as determined by the thermal denaturation method. The mean levels of DNA–DNA relatedness of the strain DMN11T with F. suzhouense XIN-1T and F. hauense BX12T were 20.5% and 29.2%, respectively. Thus, the data accumulated in this study support the suggestion that the strain DMN11T is considered to represent a novel species of the genus Flavobacterieum, for which the name Flavobacterium edaphi sp. nov. is proposed. The type strain is DMN11T (= KCTC 62114T = JCM 32372T).

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Acknowledgements

We thank Prof Dr. Bernhard Schink (University of Konstanz, Konstanz, Germany) for the suggested genus and species names. This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIT) (NRF-2017R1A2B4009448).

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Authors and Affiliations

  1. Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea

    Geeta Chhetri, Dahye Yang, Jiwon Choi, Hyungdong Kim & Taegun Seo

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  1. Geeta Chhetri
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  2. Dahye Yang
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Correspondence to Taegun Seo.

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Communicated by Erko Stackebrandt.

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Chhetri, G., Yang, D., Choi, J. et al. Flavobacterium edaphi sp. nov., isolated from soil from Jeju Island, Korea. Arch Microbiol 201, 539–545 (2019). https://doi.org/10.1007/s00203-018-1593-0

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