A novel bacterium, designated strain Acr-12T, was isolated from the coral Acropora sp. off coast of Southern Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Acr-12T belonged to the genus Endozoicomonas and had closest phylogenetic identity to Endozoicomonas acroporae Acr-14T (98.7%) and Endozoicomonas atrinae WP70T (97.8%). Cells of strain Acr-12T were Gram-negative, aerobic, non-motile, poly-β-hydroxybutyrate-accumulating, rod-shaped and formed creamy white colonies. Optimal growth occurred at 30 °C, pH 7, and in the presence of 3% NaCl. Strain Acr-12T contained summed feature 3 (C16:1ω7c and/or C16:1ω6c), summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C16:0 as the predominant fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The predominant isoprenoid quinone was Q-9. The genomic DNA G + C content was 49.6 mol%. The DNA–DNA relatedness of strain Acr-12T with respect to the closest species of the genus Endozoicomonas was less than 30%. Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Endozoicomonas. On the basis of the genotypic, chemotaxonomic, and phenotypic data, strain Acr-12T represents a novel species in the genus Endozoicomonas, for which the name Endozoicomonas coralli sp. nov. is proposed. The type strain is Acr-12T (= BCRC 80921T = KCTC 42900T).
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Appolinario LR, Tschoeke DA, Rua CPJ, Venas T, Campeão ME, Amaral GRS, Leomil L, de Oliveira L, Vieira VV, Otsuki K, Swings J, Thompson FL, Thompson CC (2016) Description of Endozoicomonas arenosclerae sp. nov. using a genomic taxonomy approach. Antonie Van Leeuwenhoek 109:431–438
Chen WM, Xie PB, Tang SL, Sheu SY (2018) Coralloluteibacterium stylophorae gen. nov., sp. nov., a new member of the family Lysobacteraceae isolated from the reef-building coral Stylophora sp. Arch Microbiol 200:473–481
Collins MD (1994) Isoprenoid quinones. In: ed Goodfellow M, O’Donnell AG (eds) Chemical methods in prokaryotic systematics. Wiley, Chichester, pp 265–309
Embley TM, Wait R (1994) Structural lipids of eubacteria. In: Goodfellow M, O’Donnell AG (eds) Chemical methods in prokaryotic systematics. Wiley, Chichester, pp 121–161
Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric DNA-DNA hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229
Garrity GM, Bell JA, Lilburn T (2005) Order VIII. Oceanospirillales ord. nov. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology (The Proteobacteria), part B (The Gammaproteobacteria), vol 2, 2nd edn. Springer, New York, pp 270–323
Hugh R, Leifson E (1953) The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66:24–26
Hyun DW, Shin NR, Kim MS, Oh SJ, Kim PS, Whon TW, Bae JW (2014) Endozoicomonas atrinae sp. nov., isolated from the intestine of a comb pen shell Atrina pectinate. Int J Syst Evol Microbiol 64:2312–2318
Kim M, Oh HS, Park SC, Chun J (2014) Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 64:346–351
Kurahashi M, Yokota A (2007) Endozoicomonas elysicola gen. nov., sp. nov., a γ-proteobacterium isolated from the sea slug Elysia ornata. Syst Appl Microbiol 30:202–206
Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
Meyer JL, Paul VJ, Teplitski M (2014) Community shifts in the surface microbiomes of the coral Porites astreoides with unusual lesions. PLoS One 9:e100316
Nishijima M, Adachi K, Katsuta A, Shizuri Y, Yamasato K (2013) Endozoicomonas numazuensis sp. nov., a gammaproteobacterium isolated from marine sponges, and emended description of the genus Endozoicomonas Kurahashi and Yokota 2007. Int J Syst Evol Microbiol 63:709–714
Nokhal TH, Schlegel HG (1983) Taxonomic study of Paracoccus denitrificans. Int J Syst Bacteriol 33:26–37
Pike RE, Haltli B, Kerr RG (2013) Description of Endozoicomonas euniceicola sp. nov. and Endozoicomonas gorgoniicola sp. nov., bacteria isolated from the octocorals Eunicea fusca and Plexaura sp., and anemended description of the genus Endozoicomonas. Int J Syst Evol Microbiol 63:4294–4302
Roder C, Bayer T, Aranda M, Kruse M, Voolstra CR (2015) Microbiome structure of the fungid coral Ctenactis echinata aligns with environmental differences. Mol Ecol 24:3501–3511
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Inc, Newark
Schreiber L, Kjeldsen KU, Obst M, Funch P, Schramm A (2006) Description of Endozoicomonas ascidiicola sp. nov., isolated from Scandinavian ascidians. Syst Appl Microbiol 39:313–318
Sheu SY, Lin KR, Hsu MY, Sheu DS, Tang SL, Chen WM (2017) Endozoicomonas acroporae sp. nov., isolated from the coral Acropora sp. Int J Syst Evol Microbiol 67:3791–3797
Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
Tindall BJ, Sikorski J, Smibert RA, Krieg NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR (eds) Methods for general and molecular bacteriology, 3rd edn. American Society for Microbiology, Washington, DC, pp 330–393
Vezzulli L, Pezzati E, Huete-Stauffer C, Pruzzo C, Cerrano C (2013) 16SrDNA pyrosequencing of the mediterranean gorgonian reveals a link among alterations in bacterial holobiont members, anthropogenic influence and disease outbreaks. PLoS One 8:e67745
Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
Yang CS, Chen MH, Arun AB, Chen CA, Wang JT, Chen WM (2010) Endozoicomonas montiporae sp. nov., isolated from the encrusting pore coral Montipora aequituberculata. Int J Syst Evol Microbiol 60:1158–1162
The authors received no specific grant from any funding agency.
Conflict of interest
The authors declare that there are no conflicts of interest.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Endozoicomonas coralli strain Acr-12T is LN879492. The Digital Protologue database Taxon Number for strain Acr-12T is TA00505.
Communicated by Erko Stackebrandt.
Electronic supplementary material
Below is the link to the electronic supplementary material.
About this article
Cite this article
Chen, WM., Lin, KR. & Sheu, SY. Endozoicomonas coralli sp. nov., isolated from the coral Acropora sp.. Arch Microbiol 201, 531–538 (2019). https://doi.org/10.1007/s00203-018-1591-2
- Endozoicomonas coralli sp. nov.
- Polyphasic taxonomy