Seonamhaeicola acroporae sp. nov., a marine species of the family Flavobacteriaceae isolated from the hard coral Acropora formosa

Abstract

A novel marine flavobacterial species, designated 3KA7-17T, was isolated from the hard coral Acropora formosa D. collected in Japan. The strain was pale-orange pigmented, Gram-stain negative, strictly aerobic, coccus shaped, and non-motile. Preliminary analysis based on the 16S rRNA gene sequence revealed an affiliation with the family Flavobacteriaceae of the phylum Bacteroidetes, and it had the greatest sequence similarity (96.0%) to Seonamhaeicola algicola Gy8T. The DNA G + C content was 34.3 mol%. MK-6 was the major menaquinone, with iso-C15:1 H and/or C13:0 3-OH (24.3%), iso-C15:0 (19.5%), iso-C15:0 3-OH (14.2%), and iso-C17:0 3-OH (15.9%) as the main (> 10%) cellular fatty acids. The major polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminolipids, and two unidentified lipids. Based on distinct phylogenetic and phenotypic evidence, the strain represents a novel species of the genus Seonamhaeicola, for which the name Seonamhaeicola acroporae sp. nov. is proposed and the type strain is 3KA7-17T (= KCTC 62713T = NBRC 113410T).

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References

  1. Alonso C, Warnecke F, Amann R, Pernthaler J (2007) High local and global diversity of Flavobacteria in marine plankton. Environ Microbiol 9:253–266

    Article  CAS  Google Scholar 

  2. Bernardet JF, Nakagawa Y, Holmes B (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070

    CAS  PubMed  Google Scholar 

  3. Colwell RR (1970) Polyphasic taxonomy of the genus Vibrio: numerical taxonomy of Vibrio cholerae, Vibrio parahaemolyticus, and related Vibrio species. J Bacteriol 104:410–433

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Fang DB, Han JR, Liu Y, Du ZJ (2017) Seonamhaeicola marinus sp. nov., isolated from marine algae. Int J Syst Evol Microbiol 67:4857–4861

    Article  CAS  PubMed  Google Scholar 

  5. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  PubMed  Google Scholar 

  6. Fitch WM (1971) Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  7. Garrity GM, Holt JG (2001) The road map to the manual. In: Boone DR, Castenholz RW, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 1, 2nd edn. Springer, New York, pp 119–166

    Google Scholar 

  8. Hansen GH, Sørheim R (1991) Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13:231–241

    Article  Google Scholar 

  9. Hertel C, Schmidt G, Fischer M, Oellers K, Hammes WP (1998) Oxygen-dependent regulation of the expression of the catalase gene katA of Lactobacillus sakei LTH677. Appl Environ Microbiol 64:1359–1365

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Jooste PJ (1985) The taxonomy and significance of Flavobacterium–Cytophaga strains from dairy sources. PhD thesis, University of the Orange Free State, South Africa

  11. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through the comparative studies of sequence evolution. J Mol Evol 16:111–120

    Article  CAS  Google Scholar 

  12. Kirchman DL (2002) The ecology of CytophagaFlavobacteria in aquatic environments. FEMS Microbiol Ecol 39:91–100

    CAS  PubMed  Google Scholar 

  13. Lewin RA, Lounsbery DM (1969) Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 58:145–170

    Article  CAS  PubMed  Google Scholar 

  14. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  15. O’Sullivan LA, Rinna J, Humphreys G, Weightman AJ, Fry JC (2006) Culturable phylogenetic diversity of the phylum ‘Bacteroidetes’ from river epilithon and coastal water and description of novel members of the family Flavobacteriaceae: Epilithonimonas tenax gen. nov., sp. nov. and Persicivirga xylanidelens gen. nov., sp. nov. Int J Syst Evol Microbiol 56:169–180

    Article  CAS  PubMed  Google Scholar 

  16. Park S, Won SM, Park DS, Yoon JH (2014) Seonamhaeicola aphaedonensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a tidal flat sediment. Int J Syst Evol Microbiol 64:1876–1881

    Article  CAS  PubMed  Google Scholar 

  17. Power DA, Johnson JA (2009) Difco™ and BBL™ manual: manual of microbiological culture media, 2nd edn. Becton Dickinson and Company, Sparks, pp 359–360

    Google Scholar 

  18. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Tamura K, Peterson D, Petersen N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Yoon J, Kang DH (2018) Terasakiella salincola sp. nov., a marine alphaproteobacterium isolated from seawater, and emended description of the genus Terasakiella. Int J Syst Evol Microbiol 68:2048–2053

    Article  PubMed  CAS  Google Scholar 

  23. Yoon J, Lee KC, Lee JS (2016) Cribrihabitans pelagius sp. nov., a marine alphaproteobacterium isolated from seawater. Int J Syst Evol Microbiol 66:3195–3200

    Article  CAS  PubMed  Google Scholar 

  24. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 67:1613–1617

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Zeng YX, Zhang F, He JF, Lee SH, Qiao ZY, Yu Y, Li HR (2013) Bacterioplankton community structure in the Arctic waters as revealed by pyrosequencing of 16S rRNA genes. Antonie Van Leeuwenhoek 103:1309–1319

    Article  PubMed  Google Scholar 

  26. Zhou YX, Du ZJ, Chen GJ (2016) Seonamhaeicola algicola sp. nov., a complex-polysaccharide-degrading bacterium isolated from Gracilaria blodgettii, and emended description of the genus Seonamhaeicola. Int J Syst Evol Microbiol 66:2064–2068

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This research was supported by the Bisa Research Grant of Keimyung University in 2018.

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Correspondence to Jaewoo Yoon.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

The digital protologue database (DPD) number for the strain 3KA7-17T is TA00563. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 3KA7-17T is LC385645.

Communicated by Erko Stackebrandt.

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Yoon, J., Yasumoto-Hirose, M. & Kasai, H. Seonamhaeicola acroporae sp. nov., a marine species of the family Flavobacteriaceae isolated from the hard coral Acropora formosa. Arch Microbiol 201, 499–504 (2019). https://doi.org/10.1007/s00203-018-1589-9

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Keywords

  • Bacteroidetes
  • Flavobacteriaceae
  • Seonamhaeicola acroporae sp. nov.
  • Acropora formosa
  • 16S rRNA gene
  • Polyphasic taxonomy