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Seonamhaeicola acroporae sp. nov., a marine species of the family Flavobacteriaceae isolated from the hard coral Acropora formosa

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Abstract

A novel marine flavobacterial species, designated 3KA7-17T, was isolated from the hard coral Acropora formosa D. collected in Japan. The strain was pale-orange pigmented, Gram-stain negative, strictly aerobic, coccus shaped, and non-motile. Preliminary analysis based on the 16S rRNA gene sequence revealed an affiliation with the family Flavobacteriaceae of the phylum Bacteroidetes, and it had the greatest sequence similarity (96.0%) to Seonamhaeicola algicola Gy8T. The DNA G + C content was 34.3 mol%. MK-6 was the major menaquinone, with iso-C15:1 H and/or C13:0 3-OH (24.3%), iso-C15:0 (19.5%), iso-C15:0 3-OH (14.2%), and iso-C17:0 3-OH (15.9%) as the main (> 10%) cellular fatty acids. The major polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminolipids, and two unidentified lipids. Based on distinct phylogenetic and phenotypic evidence, the strain represents a novel species of the genus Seonamhaeicola, for which the name Seonamhaeicola acroporae sp. nov. is proposed and the type strain is 3KA7-17T (= KCTC 62713T = NBRC 113410T).

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Acknowledgements

This research was supported by the Bisa Research Grant of Keimyung University in 2018.

Author information

Author notes

  1. Mina Yasumoto-Hirose

    Present address: Tropical Technology Plus, 12-75 Suzaki, Uruma, Okinawa, 904-2234, Japan

Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea

    Jaewoo Yoon

  2. Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan

    Mina Yasumoto-Hirose

  3. Marine Biosciences Kamaishi Research Laboratory, Kitasato University, 160-4 Utou, Okirai, Sanriku-cho, Ofunato, Iwate, 022-0101, Japan

    Hiroaki Kasai

Authors
  1. Jaewoo Yoon
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Correspondence to Jaewoo Yoon.

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The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Communicated by Erko Stackebrandt.

The digital protologue database (DPD) number for the strain 3KA7-17T is TA00563. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 3KA7-17T is LC385645.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Transmission electron micrograph of a negatively stained cell of strain 3KA7-17T. Bar, 200 nm (PPTX 9349 KB)

203_2018_1589_MOESM2_ESM.pptx

Two-dimensional thin-layer chromatograms showing the total polar lipid compositions of 3KA7-17T. Total polar lipids were detected by spraying the plate with molybdatophosphoric acid, molybdenum blue, ninhydrin, and α-naphthol. PE: phosphatidylethanolamine, AL: unidentified aminolipid, L: unidentified lipid (PPTX 780 KB)

Supplementary material 3 (XLS 48 KB)

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Yoon, J., Yasumoto-Hirose, M. & Kasai, H. Seonamhaeicola acroporae sp. nov., a marine species of the family Flavobacteriaceae isolated from the hard coral Acropora formosa. Arch Microbiol 201, 499–504 (2019). https://doi.org/10.1007/s00203-018-1589-9

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  • DOI: https://doi.org/10.1007/s00203-018-1589-9

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