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Caballeronia ginsengisoli sp. nov., isolated from ginseng cultivating soil

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Abstract

A Gram-stain-negative, strictly aerobic, non-motile, ivory colored and rod-shaped bacterium (designated Gsoil 652T) isolated from ginseng cultivating soil, was characterized using a polyphasic approach to clarify its taxonomic position. Strain Gsoil 652T was observed to grow optimally at 30 °C and at pH 7.0 on R2A agar medium. Phylogenetic analysis, based on 16S rRNA gene sequences similarities, indicated that Gsoil 652T belongs to the genus Caballeronia of the family Burkholderiaceae and was most closely related to Caballeronia choica LMG 22940T (98.9%), Caballeronia udeis LMG 27134T (98.9%), Caballeronia sordidicola LMG 22029T (98.2%) and Caballeronia humi LMG 22934T (98.1%). The DNA G+C content was 62.1 mol% and Q-8 was the major isoprenoid quinone. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified aminophospholipid, and unidentified phospholipid. The predominant fatty acids were C16:0, C17:0 cyclo and C19:0 cyclo ω8c. The DNA–DNA relatedness value between strain Gsoil 652T and closely related type strains of Caballeronia species were less than 36.0%. Moreover, strain Gsoil 652T could be distinguished phenotypically from the recognized species of the genus Caballeronia. The novel isolate, therefore, represents a novel species, for which the name Caballeronia ginsengisoli sp. nov. is proposed, with the type strain Gsoil 652T (= KACC 19441T = LMG 30326T).

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Acknowledgements

This work was supported by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment and by the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012283) “Rural Development Administration and by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Science and ICT” (2011–0031955), Republic of Korea.

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Author notes

  1. Xiao-Tian Quan and Qing-Zhen Liu contribute equally.

Authors and Affiliations

  1. Department of Biological Sciences, School of Life Sciences, Nantong University, 9 Seyuan-lu, Nantong, 226-019, Jiangsu, People’s Republic of China

    Xiao-Tian Quan

  2. Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Gyeonggi-do, 17579, Republic of Korea

    Qing-Zhen Liu, Muhammad Zubair Siddiqi & Wan-Taek Im

  3. AceEMzyme Co., Ltd., Room 403, Academic Industry Cooperation, 327 Chungang-no, Anseong-si, Kyonggi-do, 17579, Republic of Korea

    Wan-Taek Im

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  1. Xiao-Tian Quan
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  2. Qing-Zhen Liu
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  3. Muhammad Zubair Siddiqi
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  4. Wan-Taek Im
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Corresponding author

Correspondence to Wan-Taek Im.

Additional information

Communicated by Erko Stackebrandt.

The GenBank accession number for the 16S rRNA gene sequence of strain is KY078844.

The DPD taxon number for strain Gsoil 652T is TA00566.

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Supplementary Fig. S1

Transmission electron micrograph of Caballeronia ginsengisoli Gsoil 652T. Bar 2 nm. Supplementary Fig. S2. Two-dimensional TLC of polar lipids of strain Gsoil 652T. Supplementary Table S1. Negative traits of different API test kits (API ZYM, API 20NE, and API 32GN) obtained for strain Gsoil 652T. (DOCX 21410 KB)

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Quan, XT., Liu, QZ., Siddiqi, M.Z. et al. Caballeronia ginsengisoli sp. nov., isolated from ginseng cultivating soil. Arch Microbiol 201, 443–449 (2019). https://doi.org/10.1007/s00203-018-1577-0

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  • DOI: https://doi.org/10.1007/s00203-018-1577-0

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