Skip to main content
SpringerLink
Log in

Winogradskyella profunda sp. nov. isolated from the Chukchi Sea bottom sediments

Archives of Microbiology volume 201, pages 45–50 (2019)Cite this article

Abstract

An aerobic, Gram-negative, yellow-pigmented non-motile rod-shaped bacterium, designated Ch38T, was isolated from a sediment sample collected from the Chukchi Sea in the Arctic Ocean. Comparative 16S rRNA gene sequence analysis positioned strain Ch38T into the genus Winogradskyella as a distinct line adjacent to Winogradskyella multivorans KCTC 23891T, sharing the highest similarities of 97.5%, 97.2%, and 97.1% with Winogradskyella eximia KCTC 12219T, Winogradskyella damuponensis KCTC 23552T, and Winogradskyella multivorans KCTC 23891T, respectively. Strain Ch38T grew at 5–36 °C and in the presence of 1–6% (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C16:0 3-OH, anteiso-C15:0 followed by iso-C15:0 and iso-C16:1 as the major fatty acids. Polar lipids consisted of phosphatidylethanolamine, three unknown aminolipids, an unknown lipid and an unknown phospholipid. The DNA C + C content was 31.7 mol%. Based on the phylogenetic analysis and distinctive phenotypic characteristics, strain Ch38T is concluded to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella profunda sp. nov. is proposed. The type strain of the species is strain Ch38T (= KMM 9725T = KACC 19710T).

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1

References

  • Begum Z, Srinivas TNR, Manasa P, Sajlaja B, Sunil B, Prasad S, Shivaji S (2013) Winogradskyella psychrotolerans sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from Arctic sediment. Int J Syst Evol Microbiol 63:1646–1652

    Article  CAS  PubMed  Google Scholar 

  • Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surface of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868

    Article  CAS  PubMed  Google Scholar 

  • Bruns A, Rohde M, Berthe-Corti L (2001) Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006

    Article  CAS  PubMed  Google Scholar 

  • Collins MD, Shah HN (1984) Fatty acid, menaquinone and polar lipid composition of Rothia dentosacariosa. Arch Microbiol 137:247–249

    Article  CAS  Google Scholar 

  • Edwards U, Rogall T, Blöcker H, Emde M, Böttger EC (1989) Isolation and direct complete nucleotide determination of entire genes: characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fautz E, Reichenbach H (1980) A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91

    Article  CAS  Google Scholar 

  • Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

    CAS  PubMed  Google Scholar 

  • Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC

    Google Scholar 

  • Ivanova EP, Christen R, Gorshkova NM, Zhukova NV, Kurilenko VV, Crawford RJ, Mikhailov VV (2010) Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella. Int J Syst Evol Microbiol 60:1577–1580

    Article  CAS  PubMed  Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  PubMed  Google Scholar 

  • Kim M, Oh HS, Park SC, Chun J (2014) Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 64:346–351

    Article  CAS  PubMed  Google Scholar 

  • Lee DH, Cho SJ, Kim SM, Lee SB (2013) Winogradskyella damuponensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63:321–326

    Article  CAS  PubMed  Google Scholar 

  • Leifson E (1963) Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184

    CAS  PubMed  PubMed Central  Google Scholar 

  • Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118

    Article  CAS  PubMed  Google Scholar 

  • Mitchell K, Fallon RJ (1990) The determination of ubiquinone profiles by reversed-phase high performance thin-layer chromatography as an aid to the speciation of. Legionellaceae J Gen Microbiol 136:2035–2041

    Article  CAS  Google Scholar 

  • Nedashkovskaya OI, Kim SB, Han SK, Snauwaert C, Vancanneyt M, Swings J, Kim KO, Lysenko AM, Rohde M, Frolova GM, Mikhailov VV, Bae KS (2005) Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Microbiol 55:49–55

    Article  CAS  PubMed  Google Scholar 

  • Nedashkovskaya OI, Kukhlevskiy AD, Zhukova NV (2012) Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia. Int J Syst Evol Microbiol 62:1450–1456

    Article  CAS  PubMed  Google Scholar 

  • Owen J, Hill LR, Lapage SP (1969) Determination of DNA base composition from melting profiles in dilute buffers. Biopolymers 7:503–516

    Article  CAS  PubMed  Google Scholar 

  • Pearson W, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Romanenko LA, Uchino M, Frolova GM, Mikhailov VV (2007) Marixanthomonas ophiurae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from a deep-sea brittle star. Int J Syst Evol Microbiol 57:457–462

    Article  CAS  PubMed  Google Scholar 

  • Romanenko LA, Tanaka N, Svetashev VI (2013) Devosia submarina sp. nov., isolated from deep sea surface sediments. Int J Syst Evol Microbiol 63:3079–3085

    Article  CAS  PubMed  Google Scholar 

  • Sasser M (1990) Microbial identification by gas chromatographic analysis of fatty acid methyl esters (GC-FAME).Technical Note 101. MIDI, Newark

    Google Scholar 

  • Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microb Today 45:153–155

    Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Yoon JH, Lee SY (2012) Winogradskyella multivorans sp. nov., a polysaccharide-degrading bacterium isolated from seawater of an oyster farm. Antonie Van Leeuwenhoek 102:231–238

    Article  CAS  PubMed  Google Scholar 

  • Yoon BJ, Byun HD, Kim JY, Lee DH, Kang HY, Oh DC (2011) Winogradskyella lutea sp. nov., isolated from seawater, and emended description of the genus Winogradskyella. Int J Syst Evol Microbiol 61:1539–1543

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, 690022, Russia

    Lyudmila A. Romanenko, Valeriya V. Kurilenko, Konstantin V. Guzev & Valery V. Mikhailov

  2. Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia

    Vassilii I. Svetashev

Authors
  1. Lyudmila A. Romanenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Valeriya V. Kurilenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Konstantin V. Guzev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vassilii I. Svetashev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Valery V. Mikhailov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lyudmila A. Romanenko.

Ethics declarations

Conflict of interest

The authors have declared no conflict of interests.

Additional information

Communicated by Erko Stackebrandt.

The DDBJ/GenBank/EMBL accession number of the 16S rRNA gene sequence of strain KMM 9725T is LC379511. ‘Digital protologue’ database (DPD) Taxon number: TA00578.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 6166 KB)

Rights and permissions

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Romanenko, L.A., Kurilenko, V.V., Guzev, K.V. et al. Winogradskyella profunda sp. nov. isolated from the Chukchi Sea bottom sediments. Arch Microbiol 201, 45–50 (2019). https://doi.org/10.1007/s00203-018-1567-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-018-1567-2

Keywords

search

Navigation