An aerobic, Gram-negative, yellow-pigmented non-motile rod-shaped bacterium, designated Ch38T, was isolated from a sediment sample collected from the Chukchi Sea in the Arctic Ocean. Comparative 16S rRNA gene sequence analysis positioned strain Ch38T into the genus Winogradskyella as a distinct line adjacent to Winogradskyella multivorans KCTC 23891T, sharing the highest similarities of 97.5%, 97.2%, and 97.1% with Winogradskyella eximia KCTC 12219T, Winogradskyella damuponensis KCTC 23552T, and Winogradskyella multivorans KCTC 23891T, respectively. Strain Ch38T grew at 5–36 °C and in the presence of 1–6% (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C16:0 3-OH, anteiso-C15:0 followed by iso-C15:0 and iso-C16:1 as the major fatty acids. Polar lipids consisted of phosphatidylethanolamine, three unknown aminolipids, an unknown lipid and an unknown phospholipid. The DNA C + C content was 31.7 mol%. Based on the phylogenetic analysis and distinctive phenotypic characteristics, strain Ch38T is concluded to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella profunda sp. nov. is proposed. The type strain of the species is strain Ch38T (= KMM 9725T = KACC 19710T).
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Conflict of interest
The authors have declared no conflict of interests.
Communicated by Erko Stackebrandt.
The DDBJ/GenBank/EMBL accession number of the 16S rRNA gene sequence of strain KMM 9725T is LC379511. ‘Digital protologue’ database (DPD) Taxon number: TA00578.
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Romanenko, L.A., Kurilenko, V.V., Guzev, K.V. et al. Winogradskyella profunda sp. nov. isolated from the Chukchi Sea bottom sediments. Arch Microbiol 201, 45–50 (2019). https://doi.org/10.1007/s00203-018-1567-2