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Archives of Microbiology

, Volume 200, Issue 10, pp 1457–1463 | Cite as

Rhodanobacter ginsengiterrae sp. nov., an antagonistic bacterium against root rot fungal pathogen Fusarium solani, isolated from ginseng rhizospheric soil

  • Yue Huo
  • Jong-Pyo Kang
  • Jin-Kyu Park
  • Jinfeng Li
  • Ling Chen
  • Deok-Chun YangEmail author
Original Paper

Abstract

A novel bacterium, designated DCY112T, was isolated from the rhizospheric soil of a ginseng-cultivated field in Gochang-gun, Republic of Korea. Based on 16S rRNA gene sequence analysis, this isolate was assigned to the genus Rhodanobacter and is closely related to Rhodanobacter soli DCY45T (98.0%) and R. umsongensis GR24-2T (98.0%). Strain DCY112T is Gram-negative, catalase- and oxidase-positive, aerobic, non-motile, rod-shaped, and produces yellow-pigmented colonies on R2A medium. Q-8 was the predominant respiratory quinone. The major cellular fatty acids were iso-C15:0, iso-C17:0, and summed feature 9 (iso-C17:1 ω9c and/or 10-methyl-C16:0). The major polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unknown amino lipid (AL1), and an unidentified polar lipid (L3). The genomic DNA G + C content was 65.2 mol%. DNA–DNA homology values between strain DCY112T and related strains were lower than 55%. The low DNA relatedness data in combination with phenotypic and genotypic tests indicated that strain DCY112T could not be assigned to a recognized species. Strain DCY112T showed antagonistic activity against the fungal pathogen Fusarium solani (KACC 44891T), which causes ginseng root rot. The results of this study support that strain DCY112T is a novel species belonging to the genus Rhodanobacter, for which the name Rhodanobacter ginsengiterrae is proposed. The type strain is DCY112T (= KCTC 62018T = JCM 32167T).

Keywords

Rhodanobacter ginsengiterrae Rhizospheric soil Antagonistic bacteria 

Notes

Acknowledgements

This study was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (KIPET No. 317007-3), Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

203_2018_1560_MOESM1_ESM.docx (1.4 mb)
Supplementary material 1 (DOCX 1400 KB)
203_2018_1560_MOESM2_ESM.fasta (1 kb)
Supplementary material 2 (FASTA 1 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yue Huo
    • 1
  • Jong-Pyo Kang
    • 1
  • Jin-Kyu Park
    • 2
  • Jinfeng Li
    • 1
  • Ling Chen
    • 2
  • Deok-Chun Yang
    • 1
    • 2
    Email author
  1. 1.Department of Oriental Medicinal BiotechnologyKyung Hee UniversityYonginRepublic of Korea
  2. 2.Graduate School of Biotechnology, College of Life SciencesKyung Hee UniversityYonginRepublic of Korea

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