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Rhodanobacter ginsengiterrae sp. nov., an antagonistic bacterium against root rot fungal pathogen Fusarium solani, isolated from ginseng rhizospheric soil

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Abstract

A novel bacterium, designated DCY112T, was isolated from the rhizospheric soil of a ginseng-cultivated field in Gochang-gun, Republic of Korea. Based on 16S rRNA gene sequence analysis, this isolate was assigned to the genus Rhodanobacter and is closely related to Rhodanobacter soli DCY45T (98.0%) and R. umsongensis GR24-2T (98.0%). Strain DCY112T is Gram-negative, catalase- and oxidase-positive, aerobic, non-motile, rod-shaped, and produces yellow-pigmented colonies on R2A medium. Q-8 was the predominant respiratory quinone. The major cellular fatty acids were iso-C15:0, iso-C17:0, and summed feature 9 (iso-C17:1 ω9c and/or 10-methyl-C16:0). The major polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unknown amino lipid (AL1), and an unidentified polar lipid (L3). The genomic DNA G + C content was 65.2 mol%. DNA–DNA homology values between strain DCY112T and related strains were lower than 55%. The low DNA relatedness data in combination with phenotypic and genotypic tests indicated that strain DCY112T could not be assigned to a recognized species. Strain DCY112T showed antagonistic activity against the fungal pathogen Fusarium solani (KACC 44891T), which causes ginseng root rot. The results of this study support that strain DCY112T is a novel species belonging to the genus Rhodanobacter, for which the name Rhodanobacter ginsengiterrae is proposed. The type strain is DCY112T (= KCTC 62018T = JCM 32167T).

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Acknowledgements

This study was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (KIPET No. 317007-3), Republic of Korea.

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Authors and Affiliations

  1. Department of Oriental Medicinal Biotechnology, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin, Gyeonggi, 17104, Republic of Korea

    Yue Huo, Jong-Pyo Kang, Jinfeng Li & Deok-Chun Yang

  2. Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin, Gyeonggi, 17104, Republic of Korea

    Jin-Kyu Park, Ling Chen & Deok-Chun Yang

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  1. Yue Huo
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  2. Jong-Pyo Kang
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  4. Jinfeng Li
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  5. Ling Chen
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  6. Deok-Chun Yang
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Correspondence to Deok-Chun Yang.

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Additional information

Communicated by Erko Stackebrandt.

The NCBI GenBank Accession Number for the 16S rRNA gene sequence of strain DCY112T is KY694397.

Digital Protologue Database (DPD) Taxon Number TA00434.

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Huo, Y., Kang, JP., Park, JK. et al. Rhodanobacter ginsengiterrae sp. nov., an antagonistic bacterium against root rot fungal pathogen Fusarium solani, isolated from ginseng rhizospheric soil. Arch Microbiol 200, 1457–1463 (2018). https://doi.org/10.1007/s00203-018-1560-9

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  • DOI: https://doi.org/10.1007/s00203-018-1560-9

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