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Description of Lacinutrix salivirga sp. nov., a marine member of the family Flavobacteriaceae isolated from seawater

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Abstract

A novel marine bacterium, designated KMU-57T, was isolated from seawater collected from the Republic of Korea, and it was characterized using polyphasic taxonomic methods. Strain KMU-57T was Gram-stain-negative, strictly aerobic, rod-shaped, motile and dark-yellow-pigmented. Comparative analysis based on the 16S rRNA gene sequence showed the affiliation of the isolate with members of the family Flavobacteriaceae of the phylum Bacteroidetes, and it had the greatest sequence similarity (97.6%) to Lacinutrix jangbogonensis PAMC 27137T. The DNA–DNA relatedness value between strain KMU-57T and L. jangbogonensis PAMC 27137T was 37.8 ± 2.2%. The DNA G + C content of strain KMU-57T was 29.9 mol%; MK-6 was the major menaquinone with; iso-C15:1 G (18.6%) and C16:1 ω7c and/or C16:1 ω6c (15.8%) as the major (> 10%) cellular fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, three unidentified aminolipids, and five unidentified lipids. The strain represents a novel species of the genus Lacinutrix for which the name Lacinutrix salivirga sp. nov. is proposed. The type strain of L. salivirga sp. nov. is KMU-57T (= KCTC 52878T = NBRC 112845T).

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Acknowledgements

The present research was supported by the Educational–Industrial Collaboration Research Grant of YOONSUNG Co. in 2017 and by a Grant from the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program.

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Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-gu, Daegu, 42601, Republic of Korea

    Jaewoo Yoon

  2. Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea

    Jung-Sook Lee & Keun-Chul Lee

  3. Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 305-350, Republic of Korea

    Jung-Sook Lee

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  1. Jaewoo Yoon
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  2. Jung-Sook Lee
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Correspondence to Jaewoo Yoon.

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Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Communicated by Erko Stackebrandt.

The digital protologue database (DPD) number for the strain KMU-57T is TA00347. The GenBank/EMBL/DDBJ accession number of the 16S rRNA gene sequence of strain KMU-57T is LC339518.

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203_2018_1533_MOESM1_ESM.pdf

Supplementary Fig. 1. A thin-layer chromatogram showing the total polar lipid compositions of KMU-57T. Total polar lipids were detected by spraying the plate with molybdatophosphoric acid. PE: phosphatidylethanolamine, AL: unidentified aminolipid, L: unidentified lipid (PDF 54 KB)

Supplementary Fig. 2. All negative properties from the commercial test kits (PDF 51 KB)

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Yoon, J., Lee, JS. & Lee, KC. Description of Lacinutrix salivirga sp. nov., a marine member of the family Flavobacteriaceae isolated from seawater. Arch Microbiol 200, 1159–1165 (2018). https://doi.org/10.1007/s00203-018-1533-z

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  • DOI: https://doi.org/10.1007/s00203-018-1533-z

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