Cyanotrophic and arsenic oxidizing activities of Pseudomonas mendocina P6115 isolated from mine tailings containing high cyanide concentration
Mine tailings and wastewater generate man-made environments with several selective pressures, including the presence of heavy metals, arsenic and high cyanide concentrations, but severe nutritional limitations. Some oligotrophic and pioneer bacteria can colonise and grow in mine wastes containing a low concentration of organic matter and combined nitrogen sources. In this study, Pseudomonas mendocina P6115 was isolated from mine tailings in Durango, Mexico, and identified through a phylogenetic approach of 16S rRNA, gyrB, rpoB, and rpoD genes. Cell growth, cyanide consumption, and ammonia production kinetics in a medium with cyanide as sole nitrogen source showed that at the beginning, the strain grew assimilating cyanide, when cyanide was removed, ammonium was produced and accumulated in the culture medium. However, no clear stoichiometric relationship between both nitrogen sources was observed. Also, cyanide complexes were assimilated as nitrogen sources. Other phenotypic tasks that contribute to the strain’s adaptation to a mine tailing environment included siderophores production in media with moderate amounts of heavy metals, arsenite and arsenate tolerance, and the capacity of oxidizing arsenite. P. mendocina P6115 harbours cioA/cioB and aoxB genes encoding for a cyanide-insensitive oxidase and an arsenite oxidase, respectively. This is the first report where P. mendocina is described as a cyanotrophic and arsenic oxidizing species. Genotypic and phenotypic tasks of P. mendocina P6115 autochthonous from mine wastes are potentially relevant for biological treatment of residues contaminated with cyanide and arsenic.
KeywordsCyanide Arsenic Mine wastes Pseudomonas
We thank Ricardo Monterrubio-López and Luis Vázquez-Méndez for their support of determinations of metal concentration and electrical conductivity, respectively. We also thank First Majestic Plata SA de CV for the facilities for sampling. AMC thanks Consejo Nacional de Ciencia y Tecnologia (CONACYT) for the graduate scholarship awarded and Beca de Estimulo Institucional de Formación de Investigadores-Instituto Politécnico Nacional (IPN) for a scholarship complement. LVT, CHR, and JMVC are fellows of Estimulos al Desempeño de Investigadores-IPN, Comisión de Operación y Fomento de Actividades Académicas-IPN and Sistema Nacional de Investigadores-CONACYT. This work was supported by Secretaria de Investigación y Posgrado-IPN with Grants 20161850 and 20171849.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Dzombak DA, Ghosh RS, Young TC (2005) Physical–chemical properties and reactivity of cyanide in water and soil. In: Dzombak DA, Ghosh RS, Wong-Chong GM (eds) Cyanide in water and soil: chemistry, risk and management, 1st edn. CRC Press, Boca Raton, pp 57–92Google Scholar
- Grigor’eva NV, Kondrat’eva TF, Krasil’nikova EN, Karavaĭko GI (2006) Mechanism of cyanide and thiocyanate decomposition by an association of Pseudomonas putida and Pseudomonas stutzeri strains. Mikrobiologiia 75:320–328Google Scholar
- Ibáñez MI, Cabello P, Luque-Almagro VM, Sáez LP, Olaya A, Sánchez de Medina V, Luque de Castro MD, Moreno-Vivián C, Roldán MD (2017) Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters using liquid chromatography–mass spectrometry/mass spectrometry (LC–MS/MS). Plos One. https://doi.org/10.1371/journal.pone.0172908 Google Scholar
- Logsdon MJ, Hagelstein K, Mudder T (1999) The Management of cyanide in gold extraction, first ed. International council on metals and the environment. ICME, British ColumbiaGoogle Scholar
- Mekuto L, Jackson VA, Ntwampe SKO (2013) Biodegradation of free cyanide using Bacillus sp. Consortium dominated by Bacillus safensis, licheniformis and tequilensis strains: a bioprocess supported solely with whey. J Bioremed Biodeg 18:1–7Google Scholar
- Mudder TI, Botz MM (2004) Cyanide and society: a critical review. Eur J Miner Process Environ Prot 1:62–74Google Scholar
- Navarro-Noya YE, Hernández-Mendoza E, Morales-Jiménez J, Jan-Roblero J, Martínez-Romero E, Hernández-Rodríguez C (2012) Isolation and characterization of nitrogen fixing heterotrophic bacteria from the rhizosphere of pioneer plants growing on mine tailings. Appl Soil Ecol 62:52–60CrossRefGoogle Scholar
- Neff JM (1997) Ecotoxicology of arsenic in marine environment. Environ Toxicol Chem 16:917–927Google Scholar
- Relman DA (1993) Universal bacterial 16S rDNA amplification and sequencing. In: Persing DH, Smith TF, Tenover FC, White TJ (eds) Diagnostic molecular microbiDOIology: principles and applications. ASM Press, Washington, D.C, pp 489–495Google Scholar
- Secretaria de Medio Ambiente y Recursos Naturales (2004) Norma Oficial Mexicana NOM-147-SEMARNAT/SSA1-2004, que establece criterios para determinar las concentraciones de remediación de suelos contaminados por arsénico, bario, berilio, cadmio, cromo hexavalente, mercurio, níquel, plata, plomo, selenio, talio y/o vanadio. Secretaria de Medio Ambiente y Recursos Naturales, MéxicoGoogle Scholar
- Sistema Meteorológico Nacional (2013) Informe anual. http://smn.cna.gob.mx. Accesed 25 Nov 2013
- Visser WJF (1993) Contaminated land policies in some industrialised countries. Technical Soil Protection Committee (TCP), The HagueGoogle Scholar
- Vogel A, Svehla G (1979) Vogel´s textbook of macro and semimicro qualitative inorganic analysis. Longman, LondonGoogle Scholar
- Zelaya-Molina LX, Hernández-Soto LM, Guerra-Camacho JE, Monterrubio-López R, Patiño-Siciliano A, Villa-Tanaca L, Hernández-Rodríguez C (2016) Ammonia-oligotrophic and diazotrophic heavy metal-resistant Serratia liquefaciens strains from pioneer plants and mine tailings. Microb Ecol 72:324–346CrossRefPubMedGoogle Scholar
- Zeng XC, Guoji E, Wang J, Wang N, Chen X, Mu Y, Li H, Yang Y, Liu Y, Wang Y (2016) Functional characterization and unique diversity of genes and microorganisms involved in arsenite oxidation from the tailings of a realgar mine. Appl Environ Microbiol. https://doi.org/10.1128/AEM.02190-16 Google Scholar
- Zhang Z, Yin N, Cai X, Wang Z, Cui Y (2016) Arsenic redox transformation by Pseudomonas sp. HN-2 isolated from arsenic-contaminated soil in Hunan, China. J Environ Syst 47:165–173Google Scholar