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Antibacterial potential of secondary metabolites produced by Aspergillus sp., an endophyte of Mitrephora wangii

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Abstract

Mitrephora wangii, an ethnomedicinal plant, has been used as a natural antibiotic and immunity booster in Thailand. A total of 22 fungi were isolated from M. wangii flowers. The fungal isolates were categorized into six genera including Agrocybe, Aspergillus, Colletotrichum, Nigrospora, Puccinia and Ustilago. Most extracts exhibited antibacterial activity against at least one of the test bacteria. Aspergillus sp. MFLUCC16-0845 was identified as the most bioactive fungus. Chemical composition of Aspergillus sp. MFLUCC16-0845 investigated using gas chromatography–mass spectrometry indicated that the major antibacterial compound was β-thujaplicin. Moreover, the newly isolated Aspergillus sp. MFLUCC16-0845 could be exploited as a potential source of bioactive compounds and plant defense activators. In addition, it is the first time that strain of Aspergillus sp. isolated and cultured from M. wangii flowers could produce β-thujaplicin at high yield with strong antimicrobial spectrum, which may lead to wide utilization in producing cosmetics and clinical products.

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Acknowledgements

The authors are thankful to Department of Medical Science, Ministry of Health, Bangkok, Thailand for bacterial pathogens. Authors express thanks to Institute of Excellence in Fungal Research and Botanical garden of Mae Fah Luang University for collecting of isolated fungal endophytes and identifying of plants, respectively. The financial support from Mae Fah Luang University is highly appreciated.

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  1. School of Science, Mae Fah Luang University, 333 moo 1 Thasud, Muang, Chiang Rai, 57100, Thailand

    Sakon Monggoot, Tanakrit Pichaitam, Chutima Tanapichatsakul & Patcharee Pripdeevech

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  1. Sakon Monggoot
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  2. Tanakrit Pichaitam
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  3. Chutima Tanapichatsakul
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Correspondence to Patcharee Pripdeevech.

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Communicated by Erko Stackebrandt.

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Monggoot, S., Pichaitam, T., Tanapichatsakul, C. et al. Antibacterial potential of secondary metabolites produced by Aspergillus sp., an endophyte of Mitrephora wangii. Arch Microbiol 200, 951–959 (2018). https://doi.org/10.1007/s00203-018-1511-5

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  • DOI: https://doi.org/10.1007/s00203-018-1511-5

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