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Agromyces mangrovi sp. nov., a novel actinobacterium isolated from the rhizosphere of a mangrove

Archives of Microbiology volume 200, pages 939–943 (2018)Cite this article

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Abstract

A novel actinobacterium, designated HIr16-25T, was isolated from the rhizosphere soil of a mangrove growing on Iriomote Island in Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HIr16-25T fell within the cluster of the members of the genus Agromyces and the highest sequence similarity value was observed with Agromyces rhizospherae IFO 16236T (98.6%). Strain HIr16-25T possessed l-2,4-diaminobutyric acid as a diagnostic diamino acid of the peptidoglycan, and MK-12 and MK-11 as the predominant menaquinones. The major fatty acids were identified as iso-C16:0, anteiso-C15:0 and anteiso-C17:0 and the principal polar lipids were phosphatidylglycerol and one glycolipid. These chemotaxonomic features matched well those described for the members of the genus Agromyces. Meanwhile, the result of DNA–DNA hybridization and the presence of differential phenotypic characteristics between strain HIr16-25T and the type strain of A. rhizospherae indicated that strain HIr16-25T be classified as a novel species of the genus Agromyces. Therefore, we propose strain HIr16-25T to represent a novel species of the genus Agromyces, with the name Agromyces mangrovi sp. nov. The type strain is HIr16-25T (= NBRC 112812T = TBRC 7760T).

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Acknowledgements

The authors thank Dr Satoshi Sekimoto and Ms Mayuko Sukisaki (NBRC) for supporting sample collection.

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Authors and Affiliations

  1. Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan

    Moriyuki Hamada, Satomi Saitou & Tomohiko Tamura

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  1. Moriyuki Hamada
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  2. Satomi Saitou
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  3. Tomohiko Tamura
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Corresponding author

Correspondence to Moriyuki Hamada.

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Communicated by Erko Stackebrandt.

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Hamada, M., Saitou, S. & Tamura, T. Agromyces mangrovi sp. nov., a novel actinobacterium isolated from the rhizosphere of a mangrove. Arch Microbiol 200, 939–943 (2018). https://doi.org/10.1007/s00203-018-1504-4

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  • DOI: https://doi.org/10.1007/s00203-018-1504-4

Keywords

  • Actinobacteria
  • Mangrove rhizosphere
  • Agromyces
  • Polyphasic taxonomy