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Archives of Microbiology

, Volume 200, Issue 6, pp 939–943 | Cite as

Agromyces mangrovi sp. nov., a novel actinobacterium isolated from the rhizosphere of a mangrove

  • Moriyuki Hamada
  • Satomi Saitou
  • Tomohiko Tamura
Original Paper

Abstract

A novel actinobacterium, designated HIr16-25T, was isolated from the rhizosphere soil of a mangrove growing on Iriomote Island in Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HIr16-25T fell within the cluster of the members of the genus Agromyces and the highest sequence similarity value was observed with Agromyces rhizospherae IFO 16236T (98.6%). Strain HIr16-25T possessed l-2,4-diaminobutyric acid as a diagnostic diamino acid of the peptidoglycan, and MK-12 and MK-11 as the predominant menaquinones. The major fatty acids were identified as iso-C16:0, anteiso-C15:0 and anteiso-C17:0 and the principal polar lipids were phosphatidylglycerol and one glycolipid. These chemotaxonomic features matched well those described for the members of the genus Agromyces. Meanwhile, the result of DNA–DNA hybridization and the presence of differential phenotypic characteristics between strain HIr16-25T and the type strain of A. rhizospherae indicated that strain HIr16-25T be classified as a novel species of the genus Agromyces. Therefore, we propose strain HIr16-25T to represent a novel species of the genus Agromyces, with the name Agromyces mangrovi sp. nov. The type strain is HIr16-25T (= NBRC 112812T = TBRC 7760T).

Keywords

Actinobacteria Mangrove rhizosphere Agromyces Polyphasic taxonomy 

Notes

Acknowledgements

The authors thank Dr Satoshi Sekimoto and Ms Mayuko Sukisaki (NBRC) for supporting sample collection.

Supplementary material

203_2018_1504_MOESM1_ESM.pdf (246 kb)
Supplementary material 1 (PDF 246 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Biological Resource CenterNational Institute of Technology and Evaluation (NBRC)KisarazuJapan

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