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Rapid molecular identification and differentiation of common Salmonella serovars isolated from poultry, domestic animals and foodstuff using multiplex PCR assay

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Abstract

Salmonella is widely distributed throughout the world and can be found in poultry industry, animal breeding centers, food and feedstuffs of all geographical regions. This study was conducted to determine and identify Salmonella serovars isolated from poultry, calves and foodstuffs (poultry and animals products such as egg and meat). A total of one hundred isolates of Salmonella serovars including Salmonella Typhimurium, Salmonella Enteritidis, Salmonella Infantis, Salmonella Gallinarum and Salmonella Pullorum consecutively were subjected to the conventional culture, biochemical and serological assays. The utility of molecular multiplex PCR was investigated to identify and differentiate among five Salmonella serovars which were identified according to the presence of rfbJ, fljB, invA, and fliC genes in S. Typhimurium, sefA, invA and spv genes in Salmonella Enteritidis, fljB, fliC and invA genes in Salmonella Infantis, hut and slgC genes in both Salmonella Gallinarum and Salmonella Pullorum and speC gene specifically in Salmonella Gallinarum. Biochemical assays and serotyping are complicated to directly differentiate between Salmonella Gallinarum and Salmonella Pullorum because of their antigenic similarity. According to the results, Multiplex PCR can be considered as simple, rapid, accurate and useful test to identify and differentiate among Salmonella serovars.

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Acknowledgements

This work was supported by the University of Tehran, Faculty of Veterinary Medicine, Grant No. 7504002. The authors would like to thanks Mr. Iradj Ashrafi Tamai for the cooperation in laboratory process.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    A. B. Alzwghaibi, R. Yahyaraeyat, B. Nayeri Fasaei, A. Ghalyanchi Langeroudi & T. Zahraei Salehi

  2. Department of Animal Source, Faculty of Agriculture, University of Al-Qasim Green, Al-Qasim, Iraq

    A. B. Alzwghaibi

Authors
  1. A. B. Alzwghaibi
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  2. R. Yahyaraeyat
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  3. B. Nayeri Fasaei
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  4. A. Ghalyanchi Langeroudi
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  5. T. Zahraei Salehi
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Correspondence to R. Yahyaraeyat.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Communicated by Erko Stackebrandt.

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Alzwghaibi, A.B., Yahyaraeyat, R., Fasaei, B.N. et al. Rapid molecular identification and differentiation of common Salmonella serovars isolated from poultry, domestic animals and foodstuff using multiplex PCR assay. Arch Microbiol 200, 1009–1016 (2018). https://doi.org/10.1007/s00203-018-1501-7

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  • DOI: https://doi.org/10.1007/s00203-018-1501-7

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