Paucibacter aquatile sp. nov. isolated from freshwater of the Nakdong River, Republic of Korea

Abstract

A Gram-negative, aerobic, motile, and rod-shaped bacterial strain designated CR182T was isolated from freshwater of the Nakdong River, Republic of Korea. Optimal growth conditions for this novel strain were found to be: 25–30 °C, pH 6.5–8.5, and 3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicates that the strain CR182T belongs to type strains of genus Paucibacter. Strain CR182T showed 98.0% 16S rRNA gene sequence similarity with Paucibacter oligotrophus CHU3T and formed a robust phylogenetic clade with this species. The average nucleotide identity value between strain CR182T and P. oligotrophus CHU3T was 78.4% and the genome-to-genome distance was 22.2% on average. The genomic DNA G+C content calculated from the genome sequence was 66.3 mol%. Predominant cellular fatty acids of strain CR182T were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) (31.2%) and C16:0 (16.0%). Its major respiratory quinine was ubiquinone Q-8. Its polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, and two unidentified phospholipids. Its genomic DNA G+C content was 66.3%. Based on data obtained from this polyphasic taxonomic study, strain CR182T represents a novel species belonging to genus Paucibacter, for which a name of P. aquatile sp. nov. is proposed. The type strain is CR182T (= KCCM 90284T = NBRC 113032T).

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Acknowledgements

This study was supported by the NNIBR Research Program (NNIBR-2017-0000032-10) funded by the Ministry of Environment, Republic of Korea.

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Correspondence to Eu Jin Chung.

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Communicated by Erko Stackebrandt.

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Nam, Y.H., Choi, A., Hwang, J.M. et al. Paucibacter aquatile sp. nov. isolated from freshwater of the Nakdong River, Republic of Korea. Arch Microbiol 200, 877–882 (2018). https://doi.org/10.1007/s00203-018-1494-2

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Keywords

  • Paucibacter aquatile
  • Novel species
  • Polyphasic taxonomy