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Aquincola amnicola sp. nov., isolated from a freshwater river

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Abstract

Strain TTM-94T, isolated from a water sample taken from the Caohu River in Taiwan, was characterized using a polyphasic taxonomic approach. Cells of strain TTM-94T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate-accumulating, motile by a single polar flagellum, short rod-shaped and surrounded by a thick capsule and it formed cream colored colonies. Growth occurred at 20–30 °C (optimum, 30 °C), at pH 6.0–8.0 (optimum, pH 6.0), and in the presence of 0–2% NaCl (optimum 0.5%). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TTM-94T belonged to the genus Aquincola in the Rubrivivax–Roseateles–Leptothrix–Ideonella–Aquabacterium branch of the class Betaproteobacteria and its most closely related neighbour was Aquincola tertiaricarbonis L10T with sequence similarity of 97.0%. Strain TTM-94T contained summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c), C16:0 and C18:1ω7c as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized lipids. The major respiratory quinone was Q-8. Genomic DNA G + C content of strain TTM-94T was 70.7 mol%. Strain TTM-94T exhibited less than 30% DNA–DNA relatedness with A. tertiaricarbonis L10T. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain TTM-94T should be classified as a novel species of the genus Aquincola, for which the name Aquincola amnicola sp. nov. is presented. The type strain is TTM-94T (= BCRC 80890T = LMG 28709T).

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Authors and Affiliations

  1. Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung, 811, Taiwan

    Wen-Ming Chen, Yi-Ling Chen & Yi-Shu Li

  2. Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung, 811, Taiwan

    Shih-Yi Sheu

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  1. Wen-Ming Chen
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  2. Yi-Ling Chen
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Correspondence to Shih-Yi Sheu.

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Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Aquincola amnicola strain TTM-94T is LN794224. The Digital Protologue database Taxon Number for d strain TTM-94T is TA00386.

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Chen, WM., Chen, YL., Li, YS. et al. Aquincola amnicola sp. nov., isolated from a freshwater river. Arch Microbiol 200, 811–817 (2018). https://doi.org/10.1007/s00203-018-1492-4

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  • DOI: https://doi.org/10.1007/s00203-018-1492-4

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