Limoniibacter endophyticus gen. nov., sp. nov., an alphaproteobacterium isolated from the roots of Limonium otolepis

Abstract

A Gram-negative bacterium, designated as strain YIM 690229T, was isolated from the roots of Limonium otolepis. The strain was able to grow at 10–40 °C (optimum, 28–37 °C), pH 6.0–8.0 (optimum, 7.0) and in the presence of up to 7% NaCl (w/v) (optimum, up to 2.5%). Comparative 16S rRNA gene sequence analysis revealed that strain YIM 690229T shared less than 93.9% sequence similarities with members within the order Rhizobiales, and was remotely related to members of the family Hyphomicrobiaceae. Strain YIM 690229T was characterized by the presence of Q-10 as the predominant respiratory lipoquinone. The major fatty acids (> 10%) detected were C18:1 ω7c, C16:0, anteiso-C15:0 and summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B). The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanoamine and two unidentified lipids. The genomic DNA G + C content was 57.2 mol%. Data from this polyphasic taxonomy study suggested that strain YIM 690229T should be classified as a new species of a new genus within the family Hyphomicrobiaceae for which the name Limoniibacter endophyticus gen. nov., sp. nov., is proposed. The type species of the genus Limoniibacter gen. nov. is Limoniibacter endophyticus. The type strain of the species Limoniibacter endophyticus sp. nov. is YIM 690229T (= KCTC 42097T = JCM 30141T = CCTCC AB 2014130T = CGMCC 1.12906T).

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Acknowledgements

The authors are grateful to Prof. Aharon Oren for suggesting the Latin name of the species. This research was supported by Xinjiang Uygur Autonomous Region regional coordinated innovation project (Shanghai cooperation organization science and technology partnership program) (no. 2017E01031), West Light Foundation of the Chinese Academy of Sciences (nos. XBBS201305 and XBBS201201) and China Biodiversity Observation Networks (Sino BON). This research was also supported by the State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University (no. PlantKF02). W-J Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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Correspondence to Min Xiao or Wen-Jun Li.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of strain YIM 690229T is KM243752. The genus and species information have been submitted to the Digital Protologue Database under the Taxon Numbers GA00048 and TA00322, respectively.

Communicated by Erko Stackebrandt.

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Li, L., Osman, G., Salam, N. et al. Limoniibacter endophyticus gen. nov., sp. nov., an alphaproteobacterium isolated from the roots of Limonium otolepis. Arch Microbiol 200, 663–670 (2018). https://doi.org/10.1007/s00203-017-1472-0

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Keywords

  • Limoniibacter endophyticus gen. nov., sp. nov.
  • Limonium otolepis
  • Polyphasic taxonomy