Effect of Lactobacillus rhamnosus on the response of Galleria mellonella against Staphylococcus aureus and Escherichia coli infections
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This study evaluated the prophylactic effects of the live or heat-killed probiotic strain Lactobacillus rhamnosus ATCC 7469 in Galleria mellonella, inoculated with Staphylococcus aureus or Escherichia coli. L. rhamnosus suspension was prepared and a part of it was autoclaved to obtain heat-killed lactobacilli. The larvae were inoculated of these suspensions and pathogenic. The survival of the larvae was observed during 7 days and after 24 h of inoculation haemocytes counted, melanization and nitric oxide production were analyzed. Larvae survival rate increased in the group inoculated with heat-killed L. rhamnosus, however, with no statistical difference. There was a significant increase in total haemocyte counts in all test groups. Haemolymph melanization and nitric oxide production were higher in the group inoculated with L. rhamnosus and infected with S. aureus. It was concluded that, in this model of infection, heat-killed L. rhamnosus ATCC 7469 promoted greater protection in Galleria mellonella infected with S. aureus or E. coli.
KeywordsLactobacillus rhamnosus Galleria mellonella Infection Curve survival Melanization
Compliance with ethical standards
This study was not funded.
Conflict of interest
The authors declare no conflict of interest.
There is no need for ethical advice approval to use Galleria mellonella as an infection model. However, all institutional guidelines applicable to the care and use of the animals were followed.
- Dash G, Raman RP, Pani Prasad K, Makesh M, Pradeep MA, Sen S (2015) Evaluation of paraprobiotic applicability of Lactobacillus plantarum in improving the immune response and disease protection in giant freshwater prawn, Macrobrachium rosenbergii. Fish Shellfish Immunol 43(1):167 – 74. doi: 10.1016/j.fsi.2014.12.007 CrossRefPubMedGoogle Scholar
- Harb H, van Tol EA, Heine H, Braaksma M, Gross G, Overkamp K, Hennen M, Alrifai M, Conrad ML, Renz H, Garn H (2013) Neonatal supplementation of processed supernatant from Lactobacillus rhamnosus GG improves allergic airway inflammation in mice later in life. Clin Exp Allergy 43(3):353–364. doi: 10.1111/cea.12047 CrossRefGoogle Scholar
- Rajendran R, Borghi E, Falleni M, Perdoni F, Tosi D, Lappin DF, O’Donnell L, Greetham D, Ramage G, Nile C (2015) Acetylcholine protects against Candida albicans infection by inhibiting biofilm formation and promoting hemocyte function in a Galleria mellonella infection model. Eukaryot Cell 14(8):834–844. doi: 10.1128/EC.00067-15 CrossRefGoogle Scholar
- Ramarao N, Nielsen-Leroux C, Lereclus D (2012) The insect Galleria mellonella as a powerful infection model to investigate bacterial pathogenesis. J Vis Exp 70:e4392. doi: 10.3791/4392
- Semenova AD, Glazachev YI, Slepneva IA, Glupov VV (2014) Quantitative determination of nitric oxide production in haemocytes: nitrite reduction activity as a potential pathway of NO formation in haemolymph of Galleria mellonella larvae. Nitric Oxide 37:46–52. doi: 10.1016/j.niox.2013.12.011 CrossRefPubMedGoogle Scholar
- Vilela SF, Barbosa JO, Rossoni RD, Santos JD, Prata MC, Anbinder AL, Jorge AO, Junqueira JC (2015) Lactobacillus acidophilus ATCC 4356 inhibits biofilm formation by C. albicans and attenuates the experimental candidiasis in Galleria mellonella. Virulence 6(1):29–39. doi: 10.4161/21505594.2014.981486 CrossRefPubMedPubMedCentralGoogle Scholar
- Wand ME, McCowen JW, Nugent PG, Sutton JM (2013) Use of the Galleria mellonella infection model to study the opportunistic pathogen Klebsiella pneumonia supports the presence of a multifaceted interaction with the host immune response. J Med Microbiol 62(Pt 12):1790–1798. doi: 10.1099/jmm.0.063032-0 CrossRefPubMedGoogle Scholar
- WHO (2001) Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Disponível em: http://www.who.int/foodsafety/publications/fs_management/en/probiotics.pdf