Comparative analysis of microbial communities associated with bacteriomes, reproductive organs and eggs of the cicada Subpsaltria yangi
- 233 Downloads
Plant sap-feeding insects of Hemiptera often form intimate symbioses with microbes to obtain nutrients. The cicada Subpsaltria yangi is the only species of the subfamily Tettigadinae known from China. Using high-throughput sequencing combined with fluorescence in situ hybridization analysis, we characterize the bacterial composition of the bacteriomes, testes, ovaries and eggs of two representative populations of this species which occur in different habitats and feed on different plant hosts. In both populations, the bacterial community diversity in the testes was significantly higher than that in other tissues. The obligate endosymbiont Candidatus Sulcia muelleri was observed in all samples and was dominant in the bacteriomes, ovaries and eggs. The usual co-resident endosymbiont Candidatus Hodgkinia cicadicola found in some other cicadas was not detected. Instead, a novel Rhizobiales bacterium which shows a ~ 81% 16S rDNA similarity to Ca. Hodgkinia cicadicola was detected. Given that the genome of Ca. Hodgkinia cicadicola exhibits rapid evolution, it is possible that this novel Rhizobiales bacterium is a related endosymbiont with beneficial trophic functions similar to that of Ca. Hodgkinia cicadicola hosted by several certain other cicadas. The presence of the novel Rhizobiales species in other cicadas and its involvement with the adaptive evolution of related cicada hosts require further investigation. Discrepancy of bacterial communities associated with testes between the two populations may be closely related to the geographic isolation and divergence of habitats and host plants. Our results are informative for further studies of evolutionary divergence of related endosymbionts hosted in cicadas.
KeywordsCicadidae Endosymbiont Candidatus Sulcia muelleri Candidatus Hodgkinia cicadicola High-throughput sequencing Fluorescence in situ hybridization
The work was supported by the National Natural Science Foundation of China (No. 31572302) and the Chinese Universities’ Scientific Fund (Grant No. 2452017057). The authors would like to thank Mr. Zehai Hou (Northwest A&F University, China) for assistance in collecting insect specimens, and thank Mr. Yunxiang Liu (Northwest A&F University, China) for help with molecular identification of cicada eggs. The authors also would like to express our sincere thanks to Prof. John Richard Schrock (Emporia State University, USA) for revising this manuscript and providing valuable comments.
Compliance with ethical standards
Conflict of interest
We declare that we have no conflict of interest.
- Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336. doi: 10.1038/nmeth.f.303 CrossRefPubMedPubMedCentralGoogle Scholar
- Chou I, Lei Z, Li L, Lu X, Yao W (1997) The Cicadidae of China (Homoptera: Cicadoidea). Tianze Press, Hong KongGoogle Scholar
- DeSantis TZ, Hugenholtz P, Larsen N, Rojas M, Brodie EL, Keller K, Huber T, Dalevi D, Hu P, Andersen GL (2006) Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl Environ Microbiol 72:5069–5072. doi: 10.1128/AEM.03006-05 CrossRefPubMedPubMedCentralGoogle Scholar
- Kobiałka M, Michalik A, Walczak M, Junkiert Ł, Szklarzewicz T (2016) Sulcia symbiont of the leafhopper Macrosteles laevis (Ribaut, 1927) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbors Arsenophonus bacteria. Protoplasma 253:903–912. doi: 10.1007/s00709-015-0854-x CrossRefPubMedGoogle Scholar
- Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Horn DJV, Weber CF (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing bacterial communities. Appl Environ Microbiol 75:7537–7541. doi: 10.1128/AEM.01541-09 CrossRefPubMedPubMedCentralGoogle Scholar