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Kribbella sindirgiensis sp. nov. isolated from soil

Abstract

A Kribbella strain FSN23T was isolated from soil sample which was collected from Caygoren Dam lakeside located in Sındırgı, Turkey. The isolate was investigated using a polyphasic approach consisting of numeric, chemotaxonomic and molecular analysis. The isolate indicated chemotaxonomic, morphological and phylogenetic properties associated with members of the genus Kribbella. Phylogenetic analysis based on the 16S rRNA sequence of the strain demonstrated that the strain forms a subclade with K. aluminosa HKI 0478T and K. jejuensis HD9T. The organism formed an extensively branched substrate and aerial hyphae which generated spiral chains of spores with smooth surfaces. The cell wall contained ll-diaminopimelic acid, and the whole cell sugars were glucose and ribose along with trace amounts of mannose. The polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids and five unidentified polar lipids. The predominant menaquinone was MK-9(H4). The major cellular fatty acids were anteiso-C15:0 and iso-C16:0. Polyphasic taxonomy properties confirm that strain FSN23T represents a novel Kribbella taxon distinguished from closely related type strains. Hence, strain FSN23T (=KCTC 29220T = DSM 27082T) is proposed as the type strain of a novel species with the name Kribbella sindirgiensis sp. nov.

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Acknowledgements

This work was supported by Ondokuz Mayis University (OMU), Project No. PYO.FEN.1904.09.009 and we would like to thank the personnel from the DSMZ identification service for their help in chemotaxonomic analyses.

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Correspondence to Fadime Ozdemir-Kocak or Kamil Isik.

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The authors declare that they have no conflict of interest.

Additional information

The Digital Protologue database Taxon Number for strain FSN23T is TA00120. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, gyrB, rpoB, recA, relA and atpD gene sequences of Kribbella sindirgiensis FSN23T (=KCTC 29220T = DSM 27082T) are JN896614, KY581282, KY594014, KY594012, KY594013 and KY594011, respectively.

Communicated by Erko Stackebrandt.

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Fig. S1. Molybdophosphoric acid stained two-dimensional TLC of polar lipids from strain FSN23T. PG: phosphatidylglycerol, DPG: diphosphatidylglycerol, L: Lipid, PL: Unknown phospholipid (PNG 148 kb)

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Fig. S2. Scanning electron micrograph of strain FSN23T grown on ISP 2 agar at 28 °C for 28 days. Bar, 1 µm (JPEG 179 kb)

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Table S1. Sequence similarities (%) for the 16S rRNA, gyrB, rpoB recA, relA and atpD genes between FSN23T and closely related type species (DOC 39 kb)

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Table S2. GenBank/EMBL/DDBJ accession numbers of gyrB, rpoB, recA, relA and atpD sequences used in this study (DOC 58 kb)

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Table S3. MLSA and gyrB genetic distances between FSN23T and closely related type species (DOC 32 kb)

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Table S4. Fatty acids profiles of strain FSN23T and closely related type species (DOC 48 kb)

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Table S5. Cultural characteristics of the novel strain FSN23T and the most related type strains grown at 28 °C for 14 days (DOC 79 kb)

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Ozdemir-Kocak, F., Isik, K., Saricaoglu, S. et al. Kribbella sindirgiensis sp. nov. isolated from soil. Arch Microbiol 199, 1399–1407 (2017). https://doi.org/10.1007/s00203-017-1414-x

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  • DOI: https://doi.org/10.1007/s00203-017-1414-x

Keywords

  • Kribbella sindirgiensis
  • Nocardioidaceae
  • Polyphasic taxonomy
  • 16S rRNA gene