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Kribbella sindirgiensis sp. nov. isolated from soil

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Abstract

A Kribbella strain FSN23T was isolated from soil sample which was collected from Caygoren Dam lakeside located in Sındırgı, Turkey. The isolate was investigated using a polyphasic approach consisting of numeric, chemotaxonomic and molecular analysis. The isolate indicated chemotaxonomic, morphological and phylogenetic properties associated with members of the genus Kribbella. Phylogenetic analysis based on the 16S rRNA sequence of the strain demonstrated that the strain forms a subclade with K. aluminosa HKI 0478T and K. jejuensis HD9T. The organism formed an extensively branched substrate and aerial hyphae which generated spiral chains of spores with smooth surfaces. The cell wall contained ll-diaminopimelic acid, and the whole cell sugars were glucose and ribose along with trace amounts of mannose. The polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids and five unidentified polar lipids. The predominant menaquinone was MK-9(H4). The major cellular fatty acids were anteiso-C15:0 and iso-C16:0. Polyphasic taxonomy properties confirm that strain FSN23T represents a novel Kribbella taxon distinguished from closely related type strains. Hence, strain FSN23T (=KCTC 29220T = DSM 27082T) is proposed as the type strain of a novel species with the name Kribbella sindirgiensis sp. nov.

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Acknowledgements

This work was supported by Ondokuz Mayis University (OMU), Project No. PYO.FEN.1904.09.009 and we would like to thank the personnel from the DSMZ identification service for their help in chemotaxonomic analyses.

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    Authors and Affiliations

    1. Department of Nursing, School of Health, Bilecik Seyh Edebali University, 11210, Bilecik, Turkey

      Fadime Ozdemir-Kocak

    2. Biotechnology Applied and Research Center, Bilecik Seyh Edebali University, 11230, Bilecik, Turkey

      Fadime Ozdemir-Kocak

    3. Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, 55139, Samsun, Turkey

      Kamil Isik, Salih Saricaoglu, Hayrettin Saygin & Nevzat Sahin

    4. Department of Molecular Biology and Genetics, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey

      Kadriye Inan-Bektas

    5. Science Teaching Programme, Gazi Faculty of Education, Gazi University, 06500, Ankara, Turkey

      Demet Cetin

    6. Department of Biology, Faculty of Science, Anadolu University, 26470, Eskisehir, Turkey

      Kiymet Guven

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    1. Fadime Ozdemir-Kocak
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    Correspondence to Fadime Ozdemir-Kocak or Kamil Isik.

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    Additional information

    Communicated by Erko Stackebrandt.

    The Digital Protologue database Taxon Number for strain FSN23T is TA00120. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, gyrB, rpoB, recA, relA and atpD gene sequences of Kribbella sindirgiensis FSN23T (=KCTC 29220T = DSM 27082T) are JN896614, KY581282, KY594014, KY594012, KY594013 and KY594011, respectively.

    Electronic supplementary material

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    203_2017_1414_MOESM1_ESM.png

    Fig. S1. Molybdophosphoric acid stained two-dimensional TLC of polar lipids from strain FSN23T. PG: phosphatidylglycerol, DPG: diphosphatidylglycerol, L: Lipid, PL: Unknown phospholipid (PNG 148 kb)

    203_2017_1414_MOESM2_ESM.jpg

    Fig. S2. Scanning electron micrograph of strain FSN23T grown on ISP 2 agar at 28 °C for 28 days. Bar, 1 µm (JPEG 179 kb)

    203_2017_1414_MOESM3_ESM.doc

    Table S1. Sequence similarities (%) for the 16S rRNA, gyrB, rpoB recA, relA and atpD genes between FSN23T and closely related type species (DOC 39 kb)

    203_2017_1414_MOESM4_ESM.doc

    Table S2. GenBank/EMBL/DDBJ accession numbers of gyrB, rpoB, recA, relA and atpD sequences used in this study (DOC 58 kb)

    Table S3. MLSA and gyrB genetic distances between FSN23T and closely related type species (DOC 32 kb)

    Table S4. Fatty acids profiles of strain FSN23T and closely related type species (DOC 48 kb)

    203_2017_1414_MOESM7_ESM.doc

    Table S5. Cultural characteristics of the novel strain FSN23T and the most related type strains grown at 28 °C for 14 days (DOC 79 kb)

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    Ozdemir-Kocak, F., Isik, K., Saricaoglu, S. et al. Kribbella sindirgiensis sp. nov. isolated from soil. Arch Microbiol 199, 1399–1407 (2017). https://doi.org/10.1007/s00203-017-1414-x

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