A novel Gram-positive, aerobe, moderately halophilic bacterium was isolated from saline soil of Aiding lake in Xinjiang, north-west of China, designated strain YIM 98001T. Cells were rod-shaped, motile and grew at 5–20% (w/v) NaCl (optimum 10%), pH 6–10 (optimum pH 7.0) and 4–45 °C (optimum 37 °C). The major cellular fatty acids were anteiso C15:0, anteiso C17:0, iso C15:0. The predominant respiratory quinone was MK-7. Diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid were the major polar lipids. Meso-diaminopimelic acid was the diagnostic diamino acid of the cell-wall peptidoglycan. The G+C content was 36.46 mol%. 16S rRNA gene sequence analysis showed that the strain belongs to the family Bacillaceae, with the highest sequence similarity to the type strain Gracilibacillus thailandensis TP2-8T (96.84%), followed by Gracilibacillus saliphilus YIM 91119T (96.78%) and Gracilibacillus ureilyticus MF38T (96.57%), thus confirming the affiliation of strain YIM 98001T to the genus Gracilibacillus. The polyphasic approach indicates that strain YIM 98001T represents a novel species of the genus Gracilibacillus, for which the name Gracilibacillus aidingensis is proposed. The type strain is YIM 98001T (=KCTC 42683T = DSMZ 104330T).
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Ahmed I, Yokota A, Fujiwara T (2007) Gracilibacillus boraciitolerans sp. nov., a highly boron-tolerant and moderately halotolerant bacterium isolated from soil. Int J Syst Evol Microbiol 57:796–802
Chamroensaksri N, Tanasupawat S, Akaracharanya A, Visessanguan W, Kudo T, Itoh T (2010) Gracilibacillus thailandensis sp. nov., from fermented fish (pla-ra). Int J Syst Evol Microbiol 60:944–948
Chen YG, Cui XL, Pukall R, Li HM, Yang YL, Xu LH, Wen ML, Peng Q, Jiang CL (2007) Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57:2327–2332
Chen YG, Cui XL, Zhang YQ, Li WJ, Wang YX, Xu LH, Peng Q, Wen ML, Jiang CL (2008) Gracilibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from saline soil. Int J Syst Evol Microbiol 58:2403–2408
Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2, 4-diaminobutyric acid. J Appl Bacteriol 48:459–470
Cowan ST, Steel KJ (1965) Manual for the Identification of Medical Bacteria. Cambridge University Press, London
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789
Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
Gregersen T (1978) Rapid method for distinction of Gram negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127
Huo YY, Xu XW, Cui HL, Wu M (2010) Gracilibacillus ureilyticus sp. nov., a halotolerant bacterium from a saline-alkaline soil. Int J Syst Evol Microbiol 60:1383–1386
Kim P, Lee JC, Park DJ, Shin KS, Kim JY, Kim CJ (2012) Gracilibacillus bigeumensis sp. nov., a moderately halophilic bacterium from solar saltern soil. Int J Syst Evol Microbiol 62:1857–1863
Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367
Lawson PA, Deutch CE, Collins MD (1996) Phylogenetic characterization of a novel salt-tolerant Bacillus species: description of Bacillus dipsosauri sp. nov. J Appl Bacteriol 81:109–112
Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428
Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95
Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
Podestá GS, Freitas LG, Dallemole-Giaretta R, Zooca RJF, Caixeta LB, Ferraz S (2013) Meloidogyne javanica control by Pochonia chlamydosporia, Gracilibacillus dipsosauri and soil conditioner in tomato. Summa Phytopathologica 39(2):122–125
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4:406–425
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newslett 20:16
Smibert RM, Krieg NR (1981) General characterization. In: Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Philips GB (eds) Manual of methods for general bacteriology: 409–443. American Society for Microbiology, Washington, DC
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Tang SK, Wang Y, Lou K, Mao PH, Jin X, Jiang CL, Xu LH, Li WJ (2009) Gracilibacillus saliphilus sp. nov., a moderately halophilic bacterium isolated from a salt lake. Int J Syst Evol Microbiol 59:1620–1624
Tang SK, Wang Y, Guan TW, Lee JC, Kim CJ, Li WJ (2010) Amycolatopsis halophila sp. nov., a halophilic actinomycete isolated from a salt lake. Int J Syst Evol Microbiol 60:1073–1078
Tang JC, Wang M, Zhou QX, Nagata S (2011) Improved composting of Undaria pinnatifida seaweed by inoculation with Halomonas and Gracilibacillus sp. isolated from marine environments. Bioresour Technol 102(3):2925–2930
Wainø M, Tindall BJ, Schumann P, Ingvorsen K (1999) Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49:821–831
This research was supported by the National Natural Science Foundation of China (Project Nos. 31270055, 31400438), Project for Key Laboratory of Food Biotechnology of Colleges and University in Sichuan (Project No. Szjj2013-045), Key Project of Office of Education of Sichuan Province, China (Project Nos. 13205688, 13ZB0024), Technology Project of Chengdu (Project Nos. 2015-YF04-00052-JH, 2015-RC03-00033-HZ) and Key Scientific Research Fund of Xihua University (Project No. Z1220530).
The GenBank accession number for the 16S rRNA gene sequence of strain YIM 98001T is KY427823.
Communicated by Erko Stackebrandt.
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Guan, TW., Tian, L., Li, EY. et al. Gracilibacillus aidingensis sp. nov., a novel moderately halophilic bacterium isolated from Aiding salt lake. Arch Microbiol 199, 1277–1281 (2017). https://doi.org/10.1007/s00203-017-1399-5
- Gracilibacillus aidingensis sp. nov.
- Polyphasic taxonomy
- Halophilic bacterium