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Gracilibacillus aidingensis sp. nov., a novel moderately halophilic bacterium isolated from Aiding salt lake

Abstract

A novel Gram-positive, aerobe, moderately halophilic bacterium was isolated from saline soil of Aiding lake in Xinjiang, north-west of China, designated strain YIM 98001T. Cells were rod-shaped, motile and grew at 5–20% (w/v) NaCl (optimum 10%), pH 6–10 (optimum pH 7.0) and 4–45 °C (optimum 37 °C). The major cellular fatty acids were anteiso C15:0, anteiso C17:0, iso C15:0. The predominant respiratory quinone was MK-7. Diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid were the major polar lipids. Meso-diaminopimelic acid was the diagnostic diamino acid of the cell-wall peptidoglycan. The G+C content was 36.46 mol%. 16S rRNA gene sequence analysis showed that the strain belongs to the family Bacillaceae, with the highest sequence similarity to the type strain Gracilibacillus thailandensis TP2-8T (96.84%), followed by Gracilibacillus saliphilus YIM 91119T (96.78%) and Gracilibacillus ureilyticus MF38T (96.57%), thus confirming the affiliation of strain YIM 98001T to the genus Gracilibacillus. The polyphasic approach indicates that strain YIM 98001T represents a novel species of the genus Gracilibacillus, for which the name Gracilibacillus aidingensis is proposed. The type strain is YIM 98001T (=KCTC 42683T = DSMZ 104330T).

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Project Nos. 31270055, 31400438), Project for Key Laboratory of Food Biotechnology of Colleges and University in Sichuan (Project No. Szjj2013-045), Key Project of Office of Education of Sichuan Province, China (Project Nos. 13205688, 13ZB0024), Technology Project of Chengdu (Project Nos. 2015-YF04-00052-JH, 2015-RC03-00033-HZ) and Key Scientific Research Fund of Xihua University (Project No. Z1220530).

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Correspondence to Lei Tian or Shu-Kun Tang.

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The GenBank accession number for the 16S rRNA gene sequence of strain YIM 98001T is KY427823.

Communicated by Erko Stackebrandt.

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Guan, TW., Tian, L., Li, EY. et al. Gracilibacillus aidingensis sp. nov., a novel moderately halophilic bacterium isolated from Aiding salt lake. Arch Microbiol 199, 1277–1281 (2017). https://doi.org/10.1007/s00203-017-1399-5

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Keywords

  • Gracilibacillus aidingensis sp. nov.
  • Polyphasic taxonomy
  • Halophilic bacterium