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Olivibacter flavus sp. nov., a novel endophytic bacterium isolated from the root of Camellia sinensis

Abstract

A novel Gram-negative rod, endophytic bacterium, designated strain TMCC 8258T, was isolated from the root of Camellia sinensis collected from Puer, south-west China. Comparative 16S rRNA gene sequence analysis showed that the strain belongs to the family Sphingobacteriaceae and a neighbour-joining phylogenetic tree suggested that strain TMCC 8258T formed a cluster with the type strain of Olivibacter ginsengisoli (showed the highest 16S rRNA gene sequence similarity of 95.8%). Chemotaxonomic data [major fatty acid iso-C15:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), iso-C17:0 3-OH and major respiratory quinone MK-7] confirmed the affiliation of strain TMCC 8258T to the genus Olivibacter. The G + C content was 39.1 mol %. The results of the phylogenetic analysis, together with the physiological, morphological and biochemical tests, suggested that strain TMCC 8258T should be classified as representing a novel species of the genus Olivibacter, for which the name Olivibacter flavus is proposed. The type strain is TMCC 8258T (=CGMCC 1.16141 = KCTC 42683).

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 31270055, 31160003, 31270055), Project for Key Laboratory of Food Biotechnology of Colleges and University in Sichuan (Project No. Szjj2013-045), Project of Office of Education of Sichuan Province, China (Project No. 13205688, 13ZB0024) and Project of the City of Chengdu (Project No. 2015-NY02-00007-NC).

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Correspondence to Xiao-Ping Zhang or Shu-Kun Tang.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TMCC 8258T is KT328577.

Communicated by Erko Stackebrandt.

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Tian, L., Guan, TW., Yang, LL. et al. Olivibacter flavus sp. nov., a novel endophytic bacterium isolated from the root of Camellia sinensis . Arch Microbiol 199, 1237–1242 (2017). https://doi.org/10.1007/s00203-017-1391-0

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Keywords

  • Olivibacter flavus sp. nov.
  • Polyphasic taxonomy
  • Camellia sinensis