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Litorivivens aequoris sp. nov., a gammaproteobacterium isolated from seawater

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Abstract

A Gram-stain-negative, strictly aerobic, beige-pigmented, motile, chemoheterotrophic, rod-shaped or ovoid bacterium, designated strain KMU-37T, was isolated from seawater at Najeong Beach in the Republic of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the genus Litorivivens, class Gammaproteobacteria, showing highest sequence similarity (97.4%) to Litorivivens lipolytica HJTF-7T. The DNA–DNA relatedness values between strains KMU-37T and L. lipolytica HJTF-7T were 11.5 ± 0.4%. The DNA G+C content of strain KMU-37T was determined to be 53.8 mol%. Ubiquinone 8 (Q-8) was the sole respiratory quinone. The predominant cellular fatty acids were C17:1 ω8c and C16:1 ω7c and/or C16:1 ω6c. Strain KMU-37T had phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid as polar lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel species of the genus Litorivivens for which the name Litorivivens aequoris sp. nov. is proposed. The type strain of L. aequoris sp. nov. is KMU-37T (= KCCM 90262T = NBRC 111904T).

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Authors and Affiliations

  1. College of Nursing, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea

    Sangeun Jun

  2. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea

    Jaewoo Yoon

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  1. Sangeun Jun
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  2. Jaewoo Yoon
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Correspondence to Jaewoo Yoon.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Communicated by Erko Stackebrandt.

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Fig. 1

Transmission electron micrograph of a negatively stained cell of strain KMU-37T. Bar, 1 μm (PPTX 1299 kb)

Fig. 2

Thin-layer chromatograms showing the total polar lipid compositions of KMU-37T. Total polar lipids were detected by spraying the plate with molybdatophosphoric acid, molybdenum blue, α-naphthol and ninhydrin. PE: phosphatidylethanolamine, PG: phosphatidylglycerol, UL: unidentified lipid (PPTX 742 kb)

Fig. 3

Negative results from the commercial test kits.(PPTX 42 kb)

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Jun, S., Yoon, J. Litorivivens aequoris sp. nov., a gammaproteobacterium isolated from seawater. Arch Microbiol 199, 591–596 (2017). https://doi.org/10.1007/s00203-016-1329-y

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  • DOI: https://doi.org/10.1007/s00203-016-1329-y

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