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Archives of Microbiology

, Volume 199, Issue 2, pp 267–275 | Cite as

Significant relationship between soil bacterial community structure and incidence of bacterial wilt disease under continuous cropping system

  • Siyuan She
  • Jiaojiao Niu
  • Chao Zhang
  • Yunhua Xiao
  • Wu Chen
  • Linjian Dai
  • Xueduan Liu
  • Huaqun YinEmail author
Original Paper

Abstract

Soil bacteria are very important in biogeochemical cycles and play significant role in soil-borne disease suppression. Although continuous cropping is responsible for soil-borne disease enrichment, its effect on tobacco plant health and how soil bacterial communities change are yet to be elucidated. In this study, soil bacterial communities across tobacco continuous cropping time-series fields were investigated through high-throughput sequencing of 16S ribosomal RNA genes. The results showed that long-term continuous cropping could significantly alter soil microbial communities. Bacterial diversity indices and evenness indices decreased over the monoculture span and obvious variations for community structures across the three time-scale tobacco fields were detected. Compared with the first year, the abundances of Arthrobacter and Lysobacter showed a significant decrease. Besides, the abundance of the pathogen Ralstonia spp. accumulated over the monoculture span and was significantly correlated with tobacco bacterial wilt disease rate. Moreover, Pearson’s correlation demonstrated that the abundance of Arthrobacter and Lysobacter, which are considered to be beneficial bacteria had significant negative correlation with tobacco bacterial wilt disease. Therefore, after long-term continuous cropping, tobacco bacterial wilt disease could be ascribed to the alteration of the composition as well as the structure of the soil microbial community.

Keywords

Continuous cropping Microbial community structure Bacterial wilt disease 

Notes

Acknowledgments

The study was supported by National Natural Science Foundation of China (No. 31570113 and No. 41573072).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

203_2016_1301_MOESM1_ESM.docx (475 kb)
Supplementary material 1 (DOCX 474 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Siyuan She
    • 1
    • 2
  • Jiaojiao Niu
    • 1
    • 2
  • Chao Zhang
    • 3
  • Yunhua Xiao
    • 1
    • 2
  • Wu Chen
    • 3
  • Linjian Dai
    • 3
  • Xueduan Liu
    • 1
    • 2
  • Huaqun Yin
    • 1
    • 2
    Email author
  1. 1.School of Minerals Processing and BioengineeringCentral South UniversityChangshaChina
  2. 2.Key Laboratory of Biometallurgy, Ministry of EducationChangshaChina
  3. 3.College of AgronomyHunan Agricultural UniversityChangshaChina

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